IL-10 detection assay
This protocol uses an antibody specific for mouse IL- 1 0 in the E LISA method to detect mouse IL- 1 0 , and does not detect human or viral IL-10. IL-10 from ot
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Gamma interferon assay
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Detection of IL-18
Newly synthesized IL-18 is a precursor molecule to the signal peptide, which is enzymatically cleaved in order to activate it. In this ELISA method, in order to
Detection of cytokine secretion and isolation of cytokine-secreting cells by flow cytometry
This protocol is for the detection of cytokine production by activated antigen-specific T cells in vitro. If other stimulants or stimulated cells are used in vi
IL-2 and IL-4 assays
A method is described for the detection and quantification of one of the cytokines in the presence of the other using neutralizing antibodies to murine IL-2 and
Phenotypic assay of mouse macrophages
Due to the lack of characteristic surface markers of macrophages or macrophages at different stages of differentiation, macrophages are currently identified bas
Detection of α-, β- and γ-interferon-induced antiviral activity
The cells used in the assay are mouse L 929 fibroblasts, which have a high sensitivity to interferon and V S V . The cells should be derived from laboratories t
Isolation and expansion of human NK T cells
This protocol allows the identification and quantitative analysis of trace amounts of N K T cells. Effective measures should be taken to block non-specific stai
Presentation of exogenous antigens to T cells
For granular antigens or liposome-encapsulated antigens, macrophages were applied as antigen-presenting cells. Author: J.E. Colligan et al, Translator: Xuitao C