Extracellular matrix (ECM) preparation experiments
Experiments in the preparation of extracellular matrix can be used to (1) study the constituents of the extracellular matrix as well as its physiological charac
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Improvement of the Koch method of acute toxicity testing
1, study the design of acute toxicity test of chemicals, master the determination of LD50. 2. Observe the toxic reaction of phoxim.
Handling of Petri dishes and plates
The manipulation of Petri plates is necessary for cell experiments such as (1) cell growth (2) cell propagation (3) cell inoculation.
Laboratory animal grouping, labeling, and dyeing experiments
The grouping, labeling and reasonable dyeing of experimental animals are the prerequisites for obtaining good experimental results and conclusions, and are also
Operating on an open workbench
Clean and wipe the work area, place the reagent bottles, pipette, etc., on the bench (Figure 6.5), and make preparations. If necessary. If necessary, flame ste
Central nervous system culture techniques: hands-on experiments in chick embryo neuron culture
Establishing an accurate and reusable neuronal culture model in vitro is of self-evident importance for experimental research in neuroscience. Many research gro
High-throughput holistic in situ hybridization for the detection of environmental disturbances in zebrafish (Danio rerio), an embryonic tissue-specific gene expression alteration experiment
Holistic in situ hybridization is a process that allows observation of cellular gene expression (mRNA) in intact organisms. By comparing differences in gene exp
Electrical activity of single neurons: the membrane clamp technique
Over the last 20 years, the membrane clamp technique has become one of the main research tools in modern electrophysiology. Initially used only to measure singl
Experiments on optical recording techniques for culturing single neurons
Techniques for optically recording the course of activity of individual viable neurons must be considered in the light of two fundamental questions, namely, wha