Experiments on optical recording techniques for culturing single neurons
Techniques for optically recording the course of activity of individual viable neurons must be considered in the light of two fundamental questions, namely, wha
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High-throughput holistic in situ hybridization for the detection of environmental disturbances in zebrafish (Danio rerio), an embryonic tissue-specific gene expression alteration experiment
Holistic in situ hybridization is a process that allows observation of cellular gene expression (mRNA) in intact organisms. By comparing differences in gene exp
Electrical activity of single neurons: the membrane clamp technique
Over the last 20 years, the membrane clamp technique has become one of the main research tools in modern electrophysiology. Initially used only to measure singl
Experimental molecular haplotype analysis method based on emulsion polymerase chain reaction
Genotypes can be assayed by a variety of experimental methods, but assays for haplotype analysis, such as molecular haplotype analysis, are limited. Dependent o
Isolation, characterization and transplantation experiments of neural stem cells
Neural cells in the mammalian central nervous system (CNS) are derived from proliferating pluripotent stem cells or precursor cells that migrate, localize, and
Optical recording experiments of population neurons in brain slices
Optical recording involves the use of molecular indicators whose optical properties (absorption or fluorescence) vary according to the parameters of cellular ac
Experiments on the resolution of electromyographic signals in muscle fibers
The precise EMG analysis techniques covered in this chapter recover all available information in the EMG signal.EMG signal analysis consists of two categories:
Laboratory methods for the detection of chromosomal structural aberrations in human and mouse spermatozoa by fluorescence in situ hybridization
This new generation of sperm F I S H assays has identified multiple paternal risk factors such as age, polypharmacy, lifestyle, and environmental aspects and oc
Application of suppression subtractive hybridization to detect metagenomic diversity in environmental samples
Metagenomics provides information on the total genetic structure and functional composition of nondisposing samples of microbial environments without the need t