Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Bacterial cell wall degradation is a key technique that disrupts peptidoglycan and its outer structures through enzymatic, chemical, or physically assisted methods, allowing cellular contents to be released or enabling the formation of protoplasts/spheroplasts. This technology is commonly used ...
Once a cell sample enters a preservation system, the analytical result is no longer determined solely by its original biological state, but is instead jointly influenced by the anticoagulation mode, osmotic environment, buffering conditions, degree of metabolic suppression, and processing ...
Anticoagulants are auxiliary substances used to prevent blood from clotting ex vivo by inhibiting activation of coagulation factors, potentiating endogenous anticoagulant pathways, or reducing the availability of Ca²⁺.
EDTA (ethylenediaminetetraacetic acid) is ubiquitous—from blood collection tubes and cleaners to food formulations and laboratory buffers. Its hexadentate coordination and strong, broad-spectrum metal chelation underpin its use across medicine, the life sciences, analytical chemistry, and ...
Peripheral blood is one of the commonly used samples in our flow experiments. Since blood coagulates in the presence of coagulation factors after it is isolated from the body, the collection of peripheral blood samples requires the use of blood collection tubes with anticoagulants or the ...
Techniques often explored alongside immunological experiments.
