Is the mouse Fc blocker in μg packaging and is it powdery in nature? Does it dissolve in pbs?
Is the mouse Fc blocker in μg packaging and is it powdery in nature? Does it dissolve in pbs?
Topic
Flow-through Antibodies
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
77 resources · protocols, FAQs & articles
What is the difference between our Flow Antibodies available in Test packages and μg packages?
What is the difference between our Flow Antibodies available in Test packages and μg packages?
Is CD3ε a CD3?
Is CD3ε a CD3?
Is the mouse Fc blocker in μg packaging and is it powdery in nature? Does it dissolve in pbs?
Is the mouse Fc blocker in μg packaging and is it powdery in nature? Does it dissolve in pbs?
What is the difference between our Flow Antibodies available in Test packages and μg packages?
What is the difference between our Flow Antibodies available in Test packages and μg packages?
Is CD3ε a CD3?
Is CD3ε a CD3?
Flow cytometry procedure
Flow cytometry (FCM) is a single-cell quantitative analysis and sorting technique using flow cytometry. Flow cytometry is a product of highly developed and comprehensive use of monoclonal antibodies and immunocytochemistry, lasers and computer science, etc. It can effectively differentiate ...
Common Problems and Solutions for Flow Experiments
Common Problems and Solutions for Flow Experiments
Flow cytometric surface staining procedure
Flow cytometric surface staining procedure
Intranuclear Factor Staining Procedure
Intranuclear Factor Staining Procedure
Preparation and precautions for human peripheral blood PBMCs
Preparation and precautions for human peripheral blood PBMCs
Procedures and precautions for the preparation of human peripheral blood monocyte suspensions
Procedures and precautions for the preparation of human peripheral blood monocyte suspensions
Preparation of mouse ascites and single cell suspension and precautions
Preparation of mouse ascites and single cell suspension and precautions
Mouse Bone Marrow Single Cell Suspension Preparation Procedures and Precautions
Mouse Bone Marrow Single Cell Suspension Preparation Procedures and Precautions
Preparation and precautions for mouse lymph node single-cell suspensions
Preparation and precautions for mouse lymph node single-cell suspensions
Procedures and precautions for the preparation of mouse spleen single-cell suspensions
Procedures and precautions for the preparation of mouse spleen single-cell suspensions
Procedures and precautions for the preparation of mouse peripheral blood single-cell suspensions
Procedures and precautions for the preparation of mouse peripheral blood single-cell suspensions
Scenarios of application of different preparation methods for peripheral blood
Scenarios of application of different preparation methods for peripheral blood
Peripheral blood anticoagulant, EDTA or heparin?
Peripheral blood is one of the commonly used samples in our flow experiments. Since blood coagulates in the presence of coagulation factors after it is isolated from the body, the collection of peripheral blood samples requires the use of blood collection tubes with anticoagulants or the ...
What is the purpose of an isotype control? How are they selected?
What is the purpose of an isotype control? How are they selected?
Characterization and application scenarios of various enzymes in the preparation of tissue single-cell suspensions
Characterization and application scenarios of various enzymes in the preparation of tissue single-cell suspensions
Protocol for the preparation of single-cell suspensions of common tissue samples in flow experiments
Protocol for the preparation of single-cell suspensions of common tissue samples in flow experiments
Preservation methods and precautions for common flow-through samples
Preservation methods and precautions for common flow-through samples
Common sense in the purchase of flow antibodies
Common sense in the purchase of flow antibodies
Selection of fluorescent dyes for multicolor flow-through experiments
Selection of fluorescent dyes for multicolor flow-through experiments
How to Choose Flow Antibodies?
How to Choose Flow Antibodies
Methods of selecting isotype controls in flow experiments
Methods of selecting isotype controls in flow experiments
How do you really choose flow antibodies? What to look for?
How do you really choose flow antibodies? What to look for?
Selection of Mouse-Specific Cell Surface Markers
Selection of Mouse-Specific Cell Surface Markers
When do you use sterile supplies for flow-through antibody samples?
When do you use sterile supplies for flow-through antibody samples?
My samples are peripheral blood and alveolar lavage fluid from mice, how can I preserve the samples for the maximum amount of time after I remove them?
My samples are peripheral blood and alveolar lavage fluid from mice, how can I preserve the samples for the maximum amount of time after I remove them?
There are two orders of cleavage red for sample preparation, cleavage red followed by staining and staining followed by cleavage red, what to choose?
There are two orders of cleavage red for sample preparation, cleavage red followed by staining and staining followed by cleavage red, what to choose?
What are the considerations for testing tissue samples for intracellular indicators?
What are the considerations for testing tissue samples for intracellular indicators?
What are the precautions for human peripheral blood testing for intracellular factor?
What are the precautions for human peripheral blood testing for intracellular factor?
Is calcium ion-containing HBSS an option for the preparation of mouse tumor single cell suspensions?
Is calcium ion-containing HBSS an option for the preparation of mouse tumor single cell suspensions?
I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
How should I remove platelets from my blood? Can red blood cell lysate be used?
How should I remove platelets from my blood? Can red blood cell lysate be used?
I want to do a spleen flow-through, and I can't flow-through on the machine until tomorrow after picking up the sample today, how should I store the sample?
I want to do a spleen flow-through, and I can't flow-through on the machine until tomorrow after picking up the sample today, how should I store the sample?
The client's cells themselves are not fluorescent, but the drug being processed is fluorescent, how does the client go about determining what the drug is fluorescent?
The client's cells themselves are not fluorescent, but the drug being processed is fluorescent, how does the client go about determining what the drug is fluorescent?
Do I need to do a live and dead dye on my sample to eliminate dead cells? What will happen if I don't?
Do I need to do a live and dead dye on my sample to eliminate dead cells? What will happen if I don't?
I purchased three flow-through antibodies, all sized at 50 μg, how many samples can I make?
I purchased three flow-through antibodies, all sized at 50 μg, how many samples can I make?
Is the mouse Fc blocker in μg packaging and is it powdery in nature? Does it dissolve in pbs?
Is the mouse Fc blocker in μg packaging and is it powdery in nature? Does it dissolve in pbs?
How do I set up an FMO combined isotype control?
How do I set up an FMO combined isotype control?
Isoform controls help eliminate nonspecific staining, so how do you set the door?
Isoform controls help eliminate nonspecific staining, so how do you set the door?
Do I need an isotype control for all treatment groups, e.g.: 12h group, 24h group?
Do I need an isotype control for all treatment groups, e.g.: 12h group, 24h group?
I want to obtain rare cells other than red blood cells and white blood cells in my sample and keep the original cell viability of the rare cells, is this lysate suitable?
I want to obtain rare cells other than red blood cells and white blood cells in my sample and keep the original cell viability of the rare cells, is this lysate suitable?
Is it okay to dilute the rupture agent in intracellular fixation rupture agent with cell staining buffer?
Is it okay to dilute the rupture agent in intracellular fixation rupture agent with cell staining buffer?
Which is a better sealer, mouse sealer CD16/32 or unmodified mouse whole lgG antibody?
Which is a better sealer, mouse sealer CD16/32 or unmodified mouse whole lgG antibody?
I want to stain CD206 and need to break the membrane, so should I use cytokine fixative or transcription factor fixative?
I want to stain CD206 and need to break the membrane, so should I use cytokine fixative or transcription factor fixative?
Can Erythrocyte Lysate be used at room temperature?
Can Erythrocyte Lysate be used at room temperature?
Can Erythrocyte Lysate be used on avian erythrocytes?
Can Erythrocyte Lysate be used on avian erythrocytes?
If I need to label an antibody with SMCC-activated R-phycoerythrinand then use the antibody coupled with phycoerythrin as an ELISA, what other reagents are needed? Is there a specific procedure?
If I need to label an antibody with SMCC-activated R-phycoerythrinand then use the antibody coupled with phycoerythrin as an ELISA, what other reagents are needed? Is there a specific procedure?
What are the markers for sorting Raw264.7 M1 and M2 cells by flow cytometry? Can they be cultured after sorting?
What are the markers for sorting Raw264.7 M1 and M2 cells by flow cytometry? Can they be cultured after sorting?
I want to test for tumor-associated macrophages, is it okay to use only the F4/80 test?
I want to test for tumor-associated macrophages, is it okay to use only the F4/80 test?
What is the best way to choose between the different clone numbers of CD4?
What is the best way to choose between the different clone numbers of CD4?
What are the requirements of sample preparation for the use of centrifuges?
What are the requirements of sample preparation for the use of centrifuges?
What do host species and species reactivity mean, respectively?
What do host species and species reactivity mean, respectively?
Can flow antibodies be used when stored at -20°C and then thawed?
Can flow antibodies be used when stored at -20°C and then thawed?
Why centrifuge before use?
Why centrifuge before use?
The amount of cells is not 1x10^6, only 1x10^5, can the amount of antibody be reduced?
The amount of cells is not 1x10^6, only 1x10^5, can the amount of antibody be reduced?
What is the difference between our Flow Antibodies available in Test packages and μg packages?
What is the difference between our Flow Antibodies available in Test packages and μg packages?
How do I dilute the Test-packed antibodies?
How do I dilute the Test-packed antibodies?
How does the sealer work?
How does the sealer work?
How should the experimental groupings/controls be set up?
How should the experimental groupings/controls be set up?
How is the cell staining buffer used and can I use PBS instead? How much cell staining buffer do I need for one sample?
How is the cell staining buffer used and can I use PBS instead? How much cell staining buffer do I need for one sample?
What related reagents are needed for flow-through staining?
What related reagents are needed for flow-through staining?
What are the recommendations for flow assay of mouse macrophages to detect M1 and M2 types of macrophages?
What are the recommendations for flow assay of mouse macrophages to detect M1 and M2 types of macrophages?
What is the difference between E-AB-F1272D PE Anti-Mouse lL-17AAntibody[17F3] and EAB-F1199D PE Anti-Mouse lL-17AAntibody[TC11-18H10.1] are different?
What is the difference between E-AB-F1272D PE Anti-Mouse lL-17AAntibody[17F3] and EAB-F1199D PE Anti-Mouse lL-17AAntibody[TC11-18H10.1] are different?
What is the difference between CD62L and CD62?
What is the difference between CD62L and CD62?
What is the difference between Human CD45RA and CD45RO?
What is the difference between Human CD45RA and CD45RO?
What is the difference between Mouse CD90 and CD90.1 and CD90.2?
What is the difference between Mouse CD90 and CD90.1 and CD90.2?
Is CD45.1/ CD45.2 a CD45?
Is CD45.1/ CD45.2 a CD45?
Is CD3ε a CD3?
Is CD3ε a CD3?
Is CD44H a CD44?
Is CD44H a CD44?
Is CD4(domain 1) CD4?
Is CD4(domain 1) CD4?
Are CD8 and CD8α the same indicator?
Are CD8 and CD8α the same indicator?
Does the flow-through antibody to CD206 need to be fixed to break the membrane?
Does the flow-through antibody to CD206 need to be fixed to break the membrane?
Related topics
Techniques often explored alongside immunological experiments.