PCNA Mouse mAb - 1차 항체, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1

Cat. No.: Ab155826

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, See COA

Application

Flow Cytometry, IF/ICC, WB

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG1

Clone Number

C04/3D7

Conjugation

Unconjugated

개요

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

Specifications

Product Name

PCNA Mouse mAb - 1차 항체, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1

동의어

Cyclin | PCNA

사양 및 순도

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA

호스트 종

Mouse

숙주 종

마우스

특이성

PCNA

애플리케이션

WB,Flow,ELISA,ICC/IF

면역원

Full length recombinant human PCNA

포지티브 제어

WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

항체 유형

Primary antibody

복제성

Monoclonal antibodies

아이소타입

IgG1

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A purified

출처

Hybridoma supernatant

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze/thaw cycle.

이미지

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

ICC/IF : PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Ab155826) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Flow Cytometry
Flow analysis of PCNA (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. PCNA Mouse mAb (Ab155826) at 1/4000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Antibody (Ab155826) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 29.0 kDa
Observed band size: 32.0 kDa
Exposure time: 97.0 s 

적용

• 적용: Dilution info
• IF/ICC:

  0.5 μg/mL ;

• Flow Cytometry:

  0.016-2.0μg/mL ;

• WB:

  1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa. ;

관련 대상(인간)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)

Discover Target: PCNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘