PCNA Mouse mAb - 1차 항체, specific to PCNA, IgG2a

Cat. No.: Ab181179
주문 가능
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. 0.5 mg/mL
Application
Flow Cytometry, WB
Host species
마우스
Reactive species
마우스, 인간, 조언
Isotype
Mouse IgG2a
Clone Number
C12/17A3
Conjugation
Unconjugated
★
Size
USA
독일 (EU)*
Price
Qty
10μl
Ab181179-10μl
재고 있음 ≥10
—
US$45.90
50μl
Ab181179-50μl
1 재고 있음
—
US$199.90
100μl
Ab181179-100μl
1 재고 있음
—
US$299.90
1ml
Ab181179-1ml
US 주문제작 · 2–4주 ·
—
US$1,499.90
Enter a quantity for the sizes you want to add.
🧪
Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,0.5 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

개요

Application
WB: 1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa
Flow: 0.032 μg/mL - 1.0 μg/mL
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

Specifications

Product Name
PCNA Mouse mAb - 1차 항체, specific to PCNA, IgG2a
동의어
Cyclin | PCNA
사양 및 순도
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,0.5 mg/mL
숙주 종
마우스
특이성
PCNA
면역원
Full length recombinant human PCNA
포지티브 제어
WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells
변화
Unconjugated
등급
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
아이소타입
IgG2a
라이트 체인 유형
kappa
SDS-PAGE
185 kDa
정화 방법
Protein A purified
집중력
0.5 mg/mL
Product Line
biological_products
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
이미지

PCNA Mouse mAb (Ab181179) - Western Blot
All lanes: PCNA Antibody (Ab181179) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 29.0 kDa
Observed band size: 31.0 kDa
Exposure time: 54.2 s 

PCNA Mouse mAb (Ab181179) - Flow Cytometry
Overlay histogram showing HeLa cells stained with PCNA Mouse mAb (Ab181179) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

적용
적용
Dilution info
WB

1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa.

Flow Cytometry

0.032 μg/mL - 1.0 μg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

관련 대상(인간)
PCNA Tchem Proliferating cell nuclear antigen (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
작용 메커니즘
인증서(CoA, COO, BSE/TSE 및 분석 차트)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate Type날짜항목
ZJ24F0102680Certificate of AnalysisDec 16, 2025 Ab181179
ZJ24F0102681Certificate of AnalysisDec 16, 2025 Ab181179
ZJ24F0102682Certificate of AnalysisDec 16, 2025 Ab181179
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솔루션 계산기
리뷰

고객 리뷰

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

관련 상품

Goat Anti-Mouse IgG H&L Antibody - 2차 항체, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - 아이소타입 대조 항체, generated in Rat, >95%, IgG
PCNA Mouse mAb - 1차 항체, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1
Rabbit Anti-Mouse IgG H&L (HRP) - 2차 항체, IgG
Recombinant PCNA Antibody - 1차 항체, specific to PCNA, Rabbit IgG
BALB/c Mouse IgG - 아이소타입 대조 항체, IgG
Rabbit Anti-Mouse IgG antibody - 2차 항체
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체
Goat Anti-Mouse IgG H&L Antibody - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체
Rabbit Anti-Mouse IgG (HRP) - 2차 항체
Rabbit Anti-Mouse IgG (Biotin) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - 2차 항체, Goat IgG
Mouse IgG (Biotin) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (PE) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (APC) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (FITC) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG2a-Fc - 아이소타입 대조 항체, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - 2차 항체, Goat IgG
Mouse IgG2b-kappa, anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - 아이소타입 대조 항체, IgG
Mouse IgG (PerCP) - 아이소타입 대조 항체, IgG
Mouse IgG (AF405) - 아이소타입 대조 항체, IgG
Mouse IgG (AF647) - 아이소타입 대조 항체, IgG
Goat Anti-Mouse IgG H&L (AF555) - 2차 항체, specific to Mouse IgG, Goat IgG
Mouse IgG (AF700) - 아이소타입 대조 항체, IgG
Goat Anti-Mouse IgG H&L (AF700) - 2차 항체, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - 아이소타입 대조 항체
Mouse IgG (PE-Cy7) - 아이소타입 대조 항체
마우스 IgG2b-카파, 항-E8L - 아이소타입 대조 항체, specific to E8L
마우스 IgG2a-카파, 항-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - 아이소타입 대조 항체
Mouse IgG2a-Fc (FITC) - 아이소타입 대조 항체
Mouse IgG (AF555) - 아이소타입 대조 항체
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - 2차 항체, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - 1차 항체, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Recombinant PCNA Antibody - 1차 항체, specific to PCNA
Mouse IgG3-kappa (Biotin), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - 2차 항체
Goat Anti-Mouse IgG H&L (PE-Cy7) - 2차 항체
Mouse IgG (PE-Cy5.5) - 아이소타입 대조 항체
Goat Anti-Mouse IgG H&L (AF750) - 2차 항체
Mouse IgG2a-kappa (AF555), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - 아이소타입 대조 항체, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - 2차 항체
Goat Anti-Mouse IgG H&L (Oregon Green 488) - 2차 항체
Goat Anti-Mouse IgG H&L (Cy3) - 2차 항체
Mouse IgG2a-kappa (APC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG (PE-AF647) - 아이소타입 대조 항체

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