CD25 Rat mAb (PE) - Primary antibody, specific to CD25, IgG1

Cat. No.: Ab179011
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Ex:565nm ? Fluorescence excitation maximum at 565 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:575nm ? Fluorescence emission maximum at 575 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 5μL/test
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG1
Clone Number
PC-61.5.3
Conjugation
PE
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10T
Ab179011-10T
≥10
$63.90
25T
Ab179011-25T
≥10
$99.90
100T
Ab179011-100T
5
$239.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Azide Free, Ex:565nm, Em:575nm, 5μL/test Azide Free,Em:575nm,Ex:565nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application:

Flow: 5μL/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.

Specifications

Product Name
CD25 Rat mAb (PE) - Primary antibody, specific to CD25, IgG1
Synonyms
Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25, Il2r, Ly-43 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2
Specifications & Purity
ExactAb™, Validated, Azide Free, Ex:565nm, Em:575nm, 5μL/test
Host species
Rat
Specificity
CD25
Immunogen
IL-2-dependent cytolytic mouse T cell clone B6.1
Positive Control
Flow: Murine splenocytes
Conjugation
PE
Excitation (nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
Grade
Azide Free, Em:575nm, Ex:565nm, ExactAb™, Validated
Isotype
IgG1
Light Chain Type
lambda
Purification Method
Protein A High Salt
Concentration
5μL/test
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

CD25 Rat mAb (PE) (Ab179011) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (PE) (Ab179011) and CD4 Rat mAb (APC) (Ab181112) (right). BALB/c mouse splenocytes are stained with CD4 Rat mAb (APC) (Ab181112) and Rat IgG Isotype Control Antibody (PE) (Ab180312) (left).

CD25 Rat mAb (PE) (Ab179011) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (PE) (Ab179011) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).

Application
ApplicationDilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
IL2RA Tclin Interleukin-2 receptor alpha chain (2 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404890Certificate of AnalysisApr 25, 2024 Ab179011
ZJ24F0404889Certificate of AnalysisApr 25, 2024 Ab179011
ZJ24F0404888Certificate of AnalysisApr 25, 2024 Ab179011
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View Ex:565nm grade guide → View Em:575nm grade guide →

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