GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human CD276 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on. Application WB: 0.01-3 µg/mL IHC: 5-30 µg/mL Protein Function May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Specifications
Product Name
CD276 Antibody - Primary antibody, specific to CD276, Rabbit IgG
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species
Rabbit
Specificity
CD276
Immunogen
Recombinant CD276 expressed in E.coli (AA 47-219)
Positive Control
WB: HeLa, LNCAP, HEK293 and MCF7 cell lysates. IHC: Human Liver cancer tissue, Human Kidney tissue. Flow: CHO-K1 transfected with Human CD276.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified and Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
CD276 Antibody (Ab094381) - Western Blot All lanes: CD276 Antibody (Ab094381) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 90 kDa Observed band size: 92 kDa Exposure time: 71.7 s
CD276 Antibody (Ab094381) - Flow Cytometry CHO-K1 transfected with human CD276 (Stable transfection) was stained with CD276 Antibody (Ab094381) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by Cy5-conjugated Anti-rabbit IgG Secondary Antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
CD276 Antibody (Ab094381) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 μg/mL CD276 Antibody (Ab094381) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
CD276 Antibody (Ab094381) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 μg/mL CD276 Antibody (Ab094381) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Application
Application
Dilution info
IHC
5-30 µg/mL
WB
0.01-3 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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