CD86 Rat mAb (AF488) - Primärer Antikörper, specific to CD86

Cat. No.: Ab215320

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

GL1

Conjugation

AF488

Übersicht

Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry.

Specifications

Product Name

CD86 Rat mAb (AF488) - Primärer Antikörper, specific to CD86

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d

Wirtsspezies

Rat

Spezifität

CD86

Immunogen

LPS-activated CBA/Ca mouse splenic B cells

Positive Kontrolle

Flow Cytometry: C57BL/6 mouse splenocytes

Konjugation

AF488

Anregung (nm)

Blue Laser(488nm)

Emission (nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD86 Rat mAb (AF488) (Ab215320)

CD86 Rat mAb (AF488) (Ab215320) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD86 Rat mAb (AF488) (Ab215320) (red) compared to an isotype control (blue). Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Flow Cytometry: CD86 Rat mAb (AF488) (Ab215320)

CD86 Rat mAb (AF488) (Ab215320) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD86 Rat mAb (AF488) (Ab215320) (right panel) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

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CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)

Discover Target: CD86

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)