CD86 Rat mAb (AF700) - Primärer Antikörper, specific to CD86

Cat. No.: Ab215325

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

GL1

Conjugation

AF700

Übersicht

Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry.

Specifications

Product Name

CD86 Rat mAb (AF700) - Primärer Antikörper, specific to CD86

Synonyme

Activation B7-2 antigen | B70 | B7-2 antigen | B72 | B7-2 | B-lymphocyte activation antigen B7-2 | BU63 | CD28 antigen ligand 2 | CD28LG2B7-2 antigen) | CD86 antigen | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | FUN-1 | LAB72 | MGC34413 | T-lymp

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d

Wirtsspezies

Rat

Spezifität

CD86

Immunogen

LPS-activated CBA/Ca mouse splenic B cells

Positive Kontrolle

Flow: C57BL/6 mouse splenocytes

Konjugation

AF700

Anregung (nm)

Red Laser(633nm)

Emission (nm)

719nm

Ex/Em(nm)

Ex:696nm;Em:719nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD86 Rat mAb (AF700) (Ab215325)

CD86 Rat mAb (AF700) (Ab215325) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF700) (Ab215325) (red) compared to an isotype control (blue). Blue - Rat IgG (AF700) (Ab190242). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Flow Cytometry: CD86 Rat mAb (AF700) (Ab215325)

CD86 Rat mAb (AF700) (Ab215325) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF700) (Ab215325) (right panel) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF700) (Ab190242).

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Zugehörige Ziele (menschlich)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)

Discover Target: CD86

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)