QUALITÄT & REINHEITCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Validated, Carrier Free, ExactAb™, 1.0 mg/mL Carrier Free,ExactAb™,Validated für empfindliche chromatographische und analytische Workflows, die minimale Basislinieninterferenzen erfordern.
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Lagerung & Versand
Store at -20°C,Avoid repeated freezing and thawing Versand Ice chest + Ice pads. Genaue Spezifikationen finden Sie im chargenspezifischen COA.
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Qualitätsdokumente
SDS, COA, Datenblatt und Spezifikationsblatt stehen zum Download bereit. Chargenspezifische COA über Chargennummernsuche abrufbar.
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Literaturbeleg
In 0 peer-reviewten Publikationen zu Chromatographie, organischer Synthese und Kreuzkupplungsreaktionen zitiert.
Übersicht
Rabbit anti Human Cdc20 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
Spezifikationen
Product Name
Cdc20 Antibody - Primärer Antikörper, specific to Cdc20, Rabbit IgG
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC20.
Positive Kontrolle
WB: A431, THP-1, U-2 OS and C6 cell lysates. IHC: Rat brain tissue.
Konjugation
Unconjugated
Note
Carrier Free, ExactAb™, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Immunogen affinity purified
Konzentration
1.0 mg/mL
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 20.0 s
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 10.0 s
Cdc20 Antibody (Ab095575) - IHC Immunohistochemical analysis of CDC20 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the Cdc20 Antibody (Ab095575) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anwendung
Anwendung
Dilution info
IHC
1/50 - 1/100
WB
1/500-1/1000
Dokumentation
📋 Sicherheitsdatenblatt (SDS)
Umfassendes Dokument zu Gefahren, Handhabung, Lagerung und Einhaltung regulatorischer Vorschriften.
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