QUALITÄT & REINHEITExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
ExactAb™, Validated, 1.0 mg/mL ExactAb™,Validated für empfindliche chromatographische und analytische Workflows, die minimale Basislinieninterferenzen erfordern.
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Lagerung & Versand
Store at -20°C,Avoid repeated freezing and thawing Versand Ice chest + Ice pads. Genaue Spezifikationen finden Sie im chargenspezifischen COA.
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Qualitätsdokumente
SDS, COA, Datenblatt und Spezifikationsblatt stehen zum Download bereit. Chargenspezifische COA über Chargennummernsuche abrufbar.
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Literaturbeleg
In 0 peer-reviewten Publikationen zu Chromatographie, organischer Synthese und Kreuzkupplungsreaktionen zitiert.
Übersicht
Rabbit anti Human CDKN3 Antibody, Polyclonal, could be used for WB, IHC and so on. Application
WB: 1/500-1/2000 IHC: 1/100-1/300
IF/ICC: 1/200 Protein Function May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.
Spezifikationen
Product Name
CDKN3 Antibody - Primärer Antikörper, specific to CDKN3, Rabbit IgG
A synthetic peptide derived from human CDKN3 (AA 31-80).
Positive Kontrolle
WB: HEK293, Hep G2, U-2 OS, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
ExactAb™, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Immunogen affinity purified
Konzentration
1.0 mg/mL
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
CDKN3 Antibody (Ab095865) - Western Blot All lanes: CDKN3 Antibody (Ab095865) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 18, 24 kDa Exposure time: 4.5 s
CDKN3 Antibody (Ab095865) - IHC Immunohistochemical analysis of paraffin-embedded human Colon cancer. CDKN3 Antibody (Ab095865) was diluted at 1/200 (4° overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
CDKN3 Antibody (Ab095865) - IF/ICC IF analysis of CDKN3 Antibody (Ab095865) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CDKN3 Antibody (Ab095865) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Anwendung
Anwendung
Dilution info
WB
1/500-1/2000
IF/ICC
1/200
IHC
1/100-1/300
Dokumentation
📋 Sicherheitsdatenblatt (SDS)
Umfassendes Dokument zu Gefahren, Handhabung, Lagerung und Einhaltung regulatorischer Vorschriften.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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