CFA Antibody - Primärer Antikörper, specific to TBCA, Rabbit IgG

Cat. No.: Ab096146

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human CFA Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Tubulin-folding protein; involved in the early step of the tubulin folding pathway.

Specifications

Product Name

CFA Antibody - Primärer Antikörper, specific to TBCA, Rabbit IgG

Synonyme

Chaperonin cofactor A antibody | Co chaperonin associated with a & b tubulin antibody | Cofactor A antibody | tbca antibody | TBCA_HUMAN antibody | TCP1 chaperonin cofactor A antibody | TCP1-chaperonin cofactor A antibody | Tubulin cofactor a antibody | C

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 0.6 mg/mL

Wirtsspezies

Kaninchen

Spezifität

TBCA

Immunogen

Fusion protein of human CFA (AA 1-108)

Positive Kontrolle

WB: HEK293, Jurkat, THP-1 and Jurkat cell lysates. IHC: Human thyroid cancer tissue. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Antigen affinity purified

Konzentration

0.6 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: CFA Antibody (Ab096146)

CFA Antibody (Ab096146) - Western Blot
All lanes: CFA Antibody (Ab096146) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 15 kDa
Exposure time: 120.0 s

IHC: CFA Antibody (Ab096146)

CFA Antibody (Ab096146) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Tubulin Folding CFA Antibody (Ab096146). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: CFA Antibody (Ab096146)

CFA Antibody (Ab096146) - IF/ICC
IF analysis of CFA Antibody (Ab096146) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CFA Antibody (Ab096146) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Anwendung

• Anwendung: Dilution info
• IHC:

  1/50 - 1/100;

• WB:

  1/500-1/1000 ;

Zugehörige Ziele (menschlich)

TBCA Tbio Tubulin-specific chaperone A (0 Activities)

Discover Target: TBCA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CFA Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.