GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Chymotrypsin (EC 3.4.21.1), also known as α-chymotrypsin, is a proteolytic enzyme secreted by the pancreas. It exhibits endopeptidase activity, specifically hydrolyzing peptide bonds at the carboxyl terminus of aromatic amino acid residues (e.g., tyrosine and phenylalanine). Clinically, chymotrypsin is commonly used for sputum liquefaction, being effective against both purulent and non-purulent sputum, and is also employed as an adjunctive treatment for wound healing following trauma or surgery.
This kit utilizes chymotrypsin to catalyze the hydrolysis of the substrate N-succinyl-alanyl-alanyl-prolyl-phenylalanine p-nitroanilide (SAAPFpNA), releasing p-nitroaniline. This product exhibits a maximum absorption peak at 405 nm. The chymotrypsin activity can be calculated by measuring the rate of increase in absorbance.
Storage and Shipping
Storage
Store at -20°C
Shipped In
Ice chest + Ice pads
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.