Eosin, also known as eosin, is a xanthene dye in synthetic dyes. It is a pink or pink powder. Eosin is most suitable for staining with hematoxylin to show the morphological structure of normal or pathological tissues. Water soluble eosin Y contains a chromophore of quinone type benzene ring and two acidic chromophores (r-coona, r-ona) that form sodium salt. This salt forming acidic dye dissociates into negatively charged chromo acid moieties (r-coo, r-o-) when dissolved in water, that is, the colored part of the dye and the positively charged sodium ion part (na+). When staining, the negatively charged tryptophan part of Eosin Y combines with the positively charged substances in the tissue in the form of ionic bonds to develop color. Eosinystain is a special preservative, which is simple to operate and does not use toxic reagents such as mercury and methanol. It can be used for staining tissue sections or cultured cells. It can be used with immunofluorescence staining or immunohistochemical staining. On the one hand, it can be stained with immunofluorescence or other dyes after eosin staining, on the other hand, it can also be counterstained with eosin after immunohistochemical staining. This staining solution can be reused until the effect is considered to be poor, and then use a new staining solution. After staining, the cell plasma is pink or red.
Self prepared materials: 1. Distilled water 2. 4% paraformaldehyde 3. Series of ethanol
Operation steps (for reference only):
1. Sample processing a) for paraffin sections: dewaxing in xylene. Use fresh xylene and dewax. b) For frozen sections: distilled water for 2min. c) For cultured cells: fixed with paraformaldehyde. Wash with distilled water. Change to fresh distilled water and wash again.
2. Eosin staining for the above treated samples, eosin staining solution shall be used for 0.5~2min (the time shall be adjusted according to the staining results and requirements). If direct observation is needed, it can be washed twice with 70% ethanol. If dehydration, transparency and sealing are required, follow the subsequent steps. After washing with 70% ethanol, dehydration, transparency and sealing can still be carried out according to the subsequent steps. Note: if it is used for counterstaining after immunohistochemical staining, refer to the above steps to carry out eosin staining after other staining.
3. Dehydration, transparency, sealing or other staining a) dehydration, transparency, sealing: replace ethanol dehydration with fresh ethanol for dehydration. Xylene is transparent. Use fresh xylene and make it transparent again. Seal the film with neutral gum or other sealing agent. Under the microscope, the cytoplasm was pink or red. b) Other staining: if immunofluorescence staining or Hoechst and other fluorescent dyes are used, wash with ethanol after staining with eosin staining solution. PBS or normal saline or TBS or tbst and other solutions for immunostaining or fluorescent dye staining. Perform immunofluorescence staining or staining with other fluorescent dyes.
Precautions:
1. If dehydration, transparency and sealing treatment are required, xylene, neutral gum or other sealing agents shall be prepared by yourself. If the sample is paraffin sectioned, 90% ethanol, absolute ethanol and xylene should be prepared by yourself.
2. For your safety and health, please wear lab clothes and disposable gloves.
3. This product is for scientific research use only, and other uses are strictly prohibited.
K2113114