BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
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BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
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BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
It is recommended to conduct a comparison experiment, set BSA as a positive control, and add buffer at the same time as the protein sample lysate to mix and boil the denaturation, on the sample for electrophoresis to observe the bands.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?" Aladdin Knowledge Base, updated Aug 27, 2024. https://www.aladdinsci.com/us_en/faqs/bca-concentration-measured-protein-concentration-en.html
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