Bence-Jones protein characterization test
Bence-Jones protein characterization test
Ben-Zhou's Pr, also known as coagulin, is an immunoglobulin light chain or its polymer. This Pr in a certain PH conditions when heated 40 ° C a 60 ° C, precipitation occurs, the temperature increased to 100 ° C, the precipitation disappeared and then cooled when the precipitation can be reproduced.
Operation method
Ben-Chou (bence-Jones) protein characterization test
Principle
Ben-Zhou's Pr, also known as coagulin, is an immunoglobulin light chain or its polymer. This Pr in a certain PH conditions when heated 40 ° C 60 ° C, precipitation occurs, the temperature increased to 100 ° C, the precipitation disappears and then cooled when the precipitation can be reproduced. Move I. Experimental reagents: Caveat l, urine should be fresh, otherwise due to the white Pr ball Pr decomposition and denaturation and interfere with the test. 2， turbid urine can not be used, should be centrifuged and precipitated, take the supernatant urine for urine test. 3， too much of this - Zhou's Pr positive, easy to completely dissolve at 90 ℃ or more, so it is necessary to compare with the control tube can also be diluted in the original urine test. 4， boiling and filtration to remove Pr ball Pr in urine, the action should be rapid. And need to maintain high temperature. For more product details, please visit Aladdin Scientific website.
1, 200g/L sulfosalicylic acid solution.
2, 2 mol / L acetic acid buffer (PH4.9 earth 0.1).
Second, the experimental operation
l, first urine with sulfosalicylic acid method for protein characterization, such as (negative) can be a urine specimen B-J (negative).
2, take transparent urine. 4ml in a test tube and then add acetic acid buffer lml mixed, placed in water at 50 ℃ for 15 minutes, such as turbidity or precipitation, and then put the test tube into boiling water for 3 minutes, observe the precipitate in the test tube, such as precipitation does not aggravate and reduce or disappear. Tips Ben-Zhou protein positive, after boiling. Turbidity increases or precipitation increases, suggesting that there are other proteins in the urine.