DDT Mouse mAb: Key Facts, Applications & FAQs

DDT Mouse mAb is a mouse-derived monoclonal antibody specifically targeting D-dopachrome tautomerase (DDT), a member of the macrophage migration inhibitory factor (MIF) superfamily, designed for use in standard immunological research assays.

Product Name

DDT Mouse mAb

SKU

Ab326782

Host Species

Mouse

Antibody Format

Monoclonal Antibody (mAb)

Target Protein

DDT (D-dopachrome tautomerase)

Reference Clone Source

genuinbiotech #61864

What Is DDT and Why Is It Studied?

DDT, or D-dopachrome tautomerase, is a cytokine-like protein structurally and functionally related to macrophage migration inhibitory factor (MIF). It is encoded by a gene located in close proximity to MIF on chromosome 22q11.2 in humans. DDT catalyzes the tautomerization of D-dopachrome to 5,6-dihydroxyindole and shares with MIF the ability to bind the cell-surface receptor CD44 and the co-receptor CXCR2/CXCR4, implicating it in inflammatory signaling and immune regulation.

Research interest in DDT has grown due to its potential roles in cancer biology, inflammatory diseases, and as a complement to MIF-focused studies. Reliable detection tools such as the DDT Mouse mAb (Ab326782) are therefore essential for characterizing DDT expression and function in experimental models.

Antibody Characteristics and Specificity

The DDT Mouse mAb (Ab326782) is a monoclonal antibody produced in mouse and raised against the human DDT protein. As a monoclonal antibody, it offers high lot-to-lot reproducibility and defined epitope recognition, making it a consistent reagent for quantitative and qualitative studies.

Monoclonal antibodies targeting DDT must be carefully validated to confirm no cross-reactivity with the closely related MIF protein, given the structural homology between the two family members. Researchers are advised to consult the current datasheet for confirmed cross-reactivity data and validated species reactivity prior to experimental use.

Note: Detailed specificity data, clone designation, isotype, and validated application information for Ab326782 should be confirmed in the product datasheet available from Aladdin Scientific. The reference clone is genuinbiotech #61864.

Recommended Applications and Usage Guidelines

Mouse monoclonal antibodies against DDT are commonly employed in a range of standard immunological techniques. Typical applications for antibodies of this class include:

• Western Blot (WB): Detection of DDT in cell or tissue lysates under denaturing conditions.
• Immunohistochemistry (IHC): Localization of DDT protein in formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections.
• Immunofluorescence (IF): Visualization of DDT subcellular distribution in cultured cells.
• ELISA: Quantification of DDT levels in biological samples when used as a capture or detection antibody.

Optimal dilutions and conditions must be determined empirically for each application and sample type. Always refer to the current product datasheet for validated dilution ranges and positive control recommendations.

Storage, Handling, and Stability

Proper storage is critical to maintaining the activity and specificity of DDT Mouse mAb. General best practices for monoclonal antibody storage include:

• Store at –20 °C for long-term storage. Avoid repeated freeze–thaw cycles, which can lead to aggregation and loss of activity.
• For short-term use (up to 1 month), the antibody may be kept at 4 °C.
• Aliquot upon first use to minimize freeze–thaw cycles.
• Do not expose to direct light for extended periods if the product contains a conjugated fluorophore.

Important: Always refer to the certificate of analysis (CoA) and current datasheet supplied with Ab326782 for product-specific storage conditions, buffer composition, and expiry information. General guidance above reflects standard industry practice and may not reflect product-specific requirements.

Ordering Information and Support

DDT Mouse mAb is available from Aladdin Scientific under SKU Ab326782. To place an order or request a bulk quotation, visit the Aladdin Scientific website or contact our sales team directly.

For technical support, including troubleshooting western blot or IHC protocols, datasheet requests, or questions about compatibility with your experimental system, please reach out to Aladdin Scientific's technical support team. Our specialists can assist with application-specific optimization guidance.

Custom services such as conjugation, reformulation, or large-scale production inquiries can also be directed to our customer service department. Please have your SKU (Ab326782) and intended application ready when contacting support to expedite assistance.

Frequently asked questions

What is the target antigen of DDT Mouse mAb (Ab326782)?

The target antigen is DDT, also known as D-dopachrome tautomerase, a cytokine-like protein belonging to the MIF (macrophage migration inhibitory factor) superfamily. It is involved in inflammatory signaling pathways and shares structural homology with MIF.

Which experimental applications is DDT Mouse mAb validated for?

Antibodies of this class are commonly used in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA. For the specific validated applications of Ab326782, please refer to the current product datasheet available from Aladdin Scientific, as validated applications may vary by lot.

How should I store DDT Mouse mAb to maintain its performance?

For long-term storage, keep the antibody at –20 °C and avoid repeated freeze–thaw cycles by aliquoting upon first use. Short-term storage at 4 °C is acceptable for up to approximately one month. Always verify storage requirements against the CoA and datasheet provided with your specific lot of Ab326782.

Is DDT Mouse mAb cross-reactive with MIF protein?

DDT and MIF share significant structural homology as members of the same protein superfamily, making cross-reactivity a valid concern. Monoclonal antibodies are generally selected for specificity to their target epitope; however, cross-reactivity data specific to Ab326782 should be confirmed by consulting the product datasheet or contacting Aladdin Scientific technical support.

How do I order DDT Mouse mAb from Aladdin Scientific?

DDT Mouse mAb is listed under SKU Ab326782 on the Aladdin Scientific website. You can add it directly to your cart online, request a bulk quotation through the sales team, or contact customer service for assistance with institutional purchase orders and reward points or promotional pricing.

References

1. Merk M, Baugh J, Zierow S, et al. The Golgi-associated protein p115 mediates the secretion of macrophage migration inhibitory factor. J Immunol. 2009.
2. Fingerle-Rowson G, Petrenko O, Metz CN, et al. The p53-dependent apoptotic pathway is activated by macrophage migration inhibitory factor (MIF) in response to DNA damage. Proc Natl Acad Sci USA. 2003.
3. genuinbiotech product reference #61864 (DDT Mouse mAb clone source).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.