Protocols

Experiments on the effect of hemolysis and jaundice on the determination of enzyme activity

· Reviewed · Published Jun 9, 2025 · Updated Dec 24, 2024 · 8 min read

Summary

To examine the accuracy of enzyme activity measurement in the presence of interfering substances, this experiment is from the Mudanjiang Medical College undergraduate 5-year laboratory guide for laboratory tests.

Operation method

Experiments on the effect of hemolysis and jaundice on the determination of enzyme activity

Principle

L-alanine + α-ketoglutarate ALT pyruvate + L-glutamate pyruvate + NADH + H+ → L-lactate + NAD+

Materials and Instruments

Jaundice Serum
ALT/GPT reagent
Semi-automatic biochemical analyzer Test tubes Sampler

Move

1. Dilute the reagent.

2、Pre-temperature up to 37 degrees.

3、Select the measurement program．

4, test:

5. Analysis of results:

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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Aladdin Scientific. "Experiments on the effect of hemolysis and jaundice on the determination of enzyme activity" Aladdin Knowledge Base, updated Dec 24, 2024. https://www.aladdinsci.com/us_en/faqs/experiments-on-the-effect-of-hemolysis-a-en.html

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