How is the cell staining buffer used and can I use PBS instead? How much cell staining buffer do I need for one sample?
FAQs
How is the cell staining buffer used and can I use PBS instead? How much cell staining buffer do I need for one sample?
·Reviewed·Published ·Updated ·8 min read
What is the purpose of cell staining buffer and can I use PBS instead? How much cell staining buffer do I need for one sample?
The main functions of Cell Staining Buffer are to maintain cell viability, make cells stable and less sensitive to damage; maximize the intensity of fluorescent signals produced by pH-sensitive fluorescent dyes; reduce non-specific binding of antibodies to target cells; and chelate metal cations to reduce cell-to-cell adhesion. A PBS solution with 1% BSA can be substituted. One sample requires approximately 1.5 mL of Cell Staining Buffer.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Cite this article
Aladdin Scientific. "How is the cell staining buffer used and can I use PBS instead? How much cell staining buffer do I need for one sample?" Aladdin Knowledge Base, updated Aug 28, 2024. https://www.aladdinsci.com/us_en/faqs/how-is-the-cell-staining-buffer-used-en.html
Was this article helpful?YesNo3 out 5 found this helpful
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
