MGMT Mouse mAb: Detection & Research Applications

MGMT Mouse mAb is a monoclonal antibody raised in mouse, designed to specifically detect O6-methylguanine-DNA methyltransferase (MGMT), a critical DNA repair enzyme implicated in chemotherapy resistance and epigenetic regulation across multiple cancer types.

SKU

Ab351302

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS only

Target Protein

MGMT (O6-methylguanine-DNA methyltransferase)

Reference Number

absea#KC-2555

What Is MGMT and Why Does It Matter in Research?

O6-methylguanine-DNA methyltransferase (MGMT) is a conserved DNA repair protein that removes alkyl adducts from the O6 position of guanine, a lesion commonly introduced by alkylating chemotherapeutic agents such as temozolomide and carmustine. By reversing this damage in a single-step, suicidal reaction, MGMT protects genomic integrity but simultaneously confers resistance to alkylating-agent-based chemotherapy regimens.

In oncology research, MGMT is studied extensively because its promoter is frequently methylated and silenced in glioblastoma, colorectal cancer, and other tumors. Promoter methylation leads to reduced MGMT protein expression, sensitizing tumor cells to alkylating drugs and serving as a predictive biomarker for treatment response. Accurate detection of MGMT protein levels by immunological methods is therefore a cornerstone of both basic and translational cancer research.

Product Overview: MGMT Mouse mAb (Ab351302)

Aladdin Scientific offers MGMT Mouse mAb under catalog number Ab351302. This monoclonal antibody is produced in mouse and is formulated in phosphate-buffered saline (PBS) without additional carrier proteins or preservatives, making it suitable for researchers who require a carrier-free format for conjugation or specialized assay development.

The antibody is referenced under absea#KC-2555 and is intended for research use only. Its monoclonal nature ensures lot-to-lot consistency and high specificity for the MGMT target, reducing the risk of cross-reactivity with other DNA repair proteins. Researchers should validate the antibody in their specific experimental systems, as performance can vary by species, tissue type, and application.

Typical Research Applications

Mouse monoclonal antibodies targeting MGMT are commonly employed across a range of immunological and molecular biology techniques. Typical applications include:

• Western Blotting (WB): Detection of MGMT protein in cell lysates or tissue homogenates to assess expression levels across different experimental conditions.
• Immunohistochemistry (IHC): Localization of MGMT protein in formalin-fixed, paraffin-embedded (FFPE) tissue sections, particularly relevant for assessing expression in tumor biopsies.
• Immunofluorescence (IF): Visualization of MGMT subcellular localization, typically nuclear, in cultured cells.
• Immunoprecipitation (IP): Isolation of MGMT protein complexes for downstream proteomic or interaction studies.
• ELISA: Quantitative measurement of MGMT protein concentration in biological samples.

Researchers should confirm which specific applications are validated for Ab351302 by consulting the product datasheet or contacting Aladdin Scientific technical support, as validated applications may vary by lot.

Storage, Handling, and Quality Considerations

Proper storage and handling of monoclonal antibodies are essential to maintain activity and specificity over time. General best practices for PBS-formulated antibodies include:

• Store at 4°C for short-term use (up to one month) or aliquot and store at -20°C or -80°C for long-term preservation.
• Avoid repeated freeze-thaw cycles, which can denature the antibody and reduce performance. Single-use aliquots are strongly recommended.
• Keep the antibody protected from prolonged light exposure, particularly if fluorescent conjugates are prepared downstream.
• Centrifuge briefly before opening if precipitate is visible.

Because Ab351302 is formulated in PBS only (without stabilizers such as BSA or glycerol), researchers should consider adding a carrier protein or cryoprotectant if long-term storage is required. Always refer to the official product datasheet supplied with the product for lot-specific storage conditions and expiry information.

Ordering and Technical Support

MGMT Mouse mAb is available from Aladdin Scientific under SKU Ab351302. To place an order, visit the Aladdin Scientific website and search by product name or catalog number. Bulk quantities and custom formulations may be available upon request — contact the Aladdin Scientific sales team for pricing and availability.

For technical questions regarding antibody validation, recommended dilutions, compatible secondary antibodies, or troubleshooting assay performance, reach out to the Aladdin Scientific technical support team. Providing details such as your target species, sample type, and intended application will help ensure you receive the most relevant guidance.

Frequently asked questions

What is MGMT Mouse mAb used for in research?

MGMT Mouse mAb (Ab351302) is used to detect and study O6-methylguanine-DNA methyltransferase, a DNA repair enzyme central to chemotherapy resistance research and cancer biology. It may be applied in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and ELISA, depending on validated applications for the specific lot.

Why is MGMT PBS-only formulation important for my experiments?

A PBS-only formulation means the antibody contains no carrier proteins (such as BSA) or preservatives (such as sodium azide), which is advantageous when coupling the antibody to fluorophores, enzymes, or beads, or when using the antibody in assay formats sensitive to these additives. However, researchers should add a cryoprotectant if storing aliquots at -20°C or below.

How should I validate MGMT Mouse mAb in my laboratory?

Validation should include testing the antibody in your specific application (e.g., WB or IHC) using positive control samples known to express MGMT, alongside a negative control such as a MGMT-knockout cell line or isotype control antibody. Titration experiments to determine the optimal working concentration are also recommended before committing to large-scale experiments.

Is MGMT Mouse mAb suitable for use with human tissue samples?

Many anti-MGMT monoclonal antibodies are raised against conserved epitopes and show cross-reactivity with human MGMT protein; however, species reactivity for Ab351302 should be confirmed in the product datasheet. Contact Aladdin Scientific technical support if species reactivity information is not available on the datasheet.

References

1. Pegg AE. Repair of O(6)-alkylguanine by alkyltransferases. Mutat Res. 2000;462(2-3):83-100.
2. Hegi ME, et al. MGMT gene silencing and benefit from temozolomide in glioblastoma. N Engl J Med. 2005;352(10):997-1003.
3. Kaina B, et al. MGMT: key node in the battle against genotoxicity, carcinogenicity and apoptosis induced by alkylating agents. DNA Repair (Amst). 2007;6(8):1079-1099.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.