NSC 66811 (M768873): Properties, Uses & FAQs

NSC 66811 is a small-molecule compound widely referenced in oncology research as a potent disruptor of the MDM2–p53 protein–protein interaction, enabling reactivation of p53-mediated tumor suppression.

Product Name

NSC 66811

SKU

M768873

Mechanism of Action

Disrupts MDM2–p53 protein–protein interaction, restoring p53 transcriptional activity

Primary Research Application

Cancer biology; p53 pathway activation; MDM2 inhibition studies

Recommended Storage

Store at –20 °C, protected from light and moisture; verify with product datasheet

Supplier SKU Cross-reference

Also listed as Macklin M862586

What Is NSC 66811 and How Does It Work?

NSC 66811 is a synthetic small molecule that has attracted considerable interest in oncology research due to its ability to inhibit the MDM2–p53 protein–protein interaction. MDM2 (Mouse Double Minute 2) is an E3 ubiquitin ligase that negatively regulates the tumor suppressor protein p53 by promoting its proteasomal degradation. In many human cancers, MDM2 is overexpressed, leading to functional inactivation of p53.

By occupying the p53-binding pocket of MDM2, NSC 66811 displaces p53 and restores its transcriptional activity, thereby triggering downstream effects such as cell-cycle arrest and apoptosis in tumor cells that retain wild-type p53. This mechanism makes NSC 66811 a useful pharmacological tool in preclinical studies aimed at understanding p53 biology and evaluating therapeutic strategies targeting the MDM2–p53 axis.

Solubility, Formulation and Handling Guidelines

Researchers should consult the official Aladdin product datasheet for SKU M768873 for confirmed physicochemical data including molecular weight, purity, and solubility parameters. As a general guideline for structurally similar MDM2 inhibitors, DMSO is commonly used as a primary solvent for stock solution preparation. It is advisable to prepare stock solutions at a concentration that allows subsequent dilution to a final DMSO concentration of ≤0.1% in cell-based assays to minimize cytotoxic solvent effects.

Prior to use, briefly vortex and, if necessary, sonicate the solution to ensure complete dissolution. Avoid repeated freeze–thaw cycles by preparing single-use aliquots. All handling should be performed in accordance with the Safety Data Sheet (SDS) for this compound.

Storage Conditions and Stability

To maintain compound integrity, NSC 66811 (M768873) should be stored at –20 °C in a tightly sealed container, protected from light and moisture. Lyophilized or solid-form compounds are generally more stable than solutions; therefore, it is recommended to prepare working solutions freshly or store aliquots at –80 °C for short-term use.

Always verify specific storage requirements and shelf-life information on the current product datasheet or certificate of analysis (CoA) supplied with each lot. If any visible changes in color, clarity or precipitate formation are observed, the compound should not be used without re-verification of identity and purity.

Recommended Research Applications

NSC 66811 is primarily employed as a biochemical and cell biology tool in the following research contexts:

• MDM2–p53 interaction assays: Competitive binding assays (e.g., fluorescence polarization, SPR) to characterize inhibitor potency.
• Cell viability and apoptosis studies: Evaluation of p53-dependent apoptotic responses in wild-type p53 cancer cell lines.
• Gene expression profiling: Examining transcriptional targets of p53 upon MDM2 inhibition.
• Combination studies: Used alongside chemotherapeutic agents to assess synergistic effects on tumor cell killing.

NSC 66811 is intended for research use only and is not approved for human therapeutic use.

Ordering, Cross-References and Technical Support

NSC 66811 is available from Aladdin Scientific under SKU M768873. The same compound may also be listed by partner supplier Macklin under catalog number M862586. When ordering, always quote the Aladdin SKU to ensure you receive the correct product and associated documentation.

For technical queries regarding purity specifications, certificate of analysis requests, custom packaging, or bulk pricing, please contact Aladdin Scientific's technical support team. Regulatory and safety documentation (SDS) can be downloaded directly from the product page.

Frequently asked questions

What is the mechanism of action of NSC 66811?

NSC 66811 acts as a small-molecule inhibitor of the MDM2–p53 protein–protein interaction. It binds to the p53-binding domain of MDM2, preventing MDM2 from targeting p53 for proteasomal degradation and thereby restoring p53 transcriptional activity and its tumor-suppressive functions.

What solvent is recommended for dissolving NSC 66811?

DMSO is the most commonly used solvent for dissolving structurally similar MDM2 inhibitors and is generally recommended for preparing stock solutions of NSC 66811. Ensure that the final DMSO concentration in cell-based experiments does not exceed 0.1% to avoid solvent-related cytotoxicity. Please confirm solubility details in the official Aladdin product datasheet for M768873.

How should NSC 66811 be stored to ensure stability?

NSC 66811 (M768873) should be stored at –20 °C in a tightly sealed container, away from light and moisture. To avoid repeated freeze–thaw cycles that can degrade compound activity, prepare single-use aliquots. Always refer to the certificate of analysis and product datasheet supplied with your lot for authoritative storage instructions.

Is NSC 66811 suitable for in vivo studies?

NSC 66811 has been used as an in vitro tool compound to study MDM2–p53 interactions. Its suitability for in vivo studies depends on its pharmacokinetic properties (absorption, distribution, metabolism, excretion), which should be evaluated experimentally. Researchers planning in vivo work should review available literature and consult the datasheet; Aladdin Scientific's technical support team can assist with formulation guidance.

What is the Aladdin SKU for NSC 66811 and are there cross-references to other suppliers?

The Aladdin Scientific SKU for NSC 66811 is M768873. The compound is also referenced under Macklin catalog number M862586. When ordering or referencing this compound, always use the Aladdin SKU M768873 to ensure correct product identification and documentation retrieval.

References

1. Vassilev L T et al. In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science. 2004;303(5659):844-848. doi:10.1126/science.1092472
2. Shangary S, Wang S. Small-molecule inhibitors of the MDM2-p53 protein-protein interaction to reactivate p53 function: a novel approach for cancer therapy. Annu Rev Pharmacol Toxicol. 2009;49:223-241. doi:10.1146/annurev.pharmtox.48.113006.094723
3. Aladdin Scientific. NSC 66811 Product Datasheet, SKU M768873. Aladdin Scientific, Shanghai.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.