p95 Mouse mAb Usage Guidelines | Aladdin Scientific

p95 Mouse mAb is a mouse-derived monoclonal antibody supplied in PBS formulation (Aladdin Cat. Ab350605, sourced as absea #KC-1341) designed for reliable detection of p95 target protein in standard immunological assays.

Catalog Number

Ab350605

Source Reference

absea #KC-1341

Antibody Type

Mouse Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline) Only

Target

p95 protein

Species Reactivity

To be confirmed; refer to product datasheet

Product Overview

p95 Mouse mAb (Aladdin Cat. No. Ab350605; absea #KC-1341) is a mouse-derived monoclonal antibody formulated in phosphate-buffered saline (PBS). The PBS-only formulation ensures compatibility with a wide range of downstream immunoassay workflows and avoids interference from carrier proteins such as BSA or glycerol that could affect certain assay formats. Researchers should consult the product datasheet for confirmed species reactivity, immunogen details, isotype, and specific validated applications.

Storage and Handling

Proper storage is critical to maintaining antibody activity and specificity. The following general guidelines apply to PBS-formulated monoclonal antibodies such as p95 Mouse mAb:

• Short-term storage (up to 1 month): Store at 4 °C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot the antibody into single-use volumes and store at −20 °C or −80 °C to preserve activity.
• Avoid: Storage at room temperature for extended periods, exposure to direct light, and repeated freeze-thaw cycles, all of which can degrade antibody performance.
• Carrier protein addition: If long-term stability is a concern, consult the datasheet or Aladdin technical support regarding the suitability of adding a stabilising carrier protein such as BSA (0.1% final concentration) to the PBS-only formulation.

Always refer to the specific lot certificate of analysis (CoA) for storage conditions confirmed by the manufacturer.

Recommended Workflow and Dilution Guidelines

Because the specific validated applications for p95 Mouse mAb (Ab350605) are detailed in the manufacturer datasheet, researchers should treat the following as general best-practice guidance for PBS-formulated mouse monoclonal antibodies pending confirmation from the product CoA:

1. Centrifuge before use: Briefly spin the vial (300 × g, 5 seconds) to collect any liquid from the cap before opening.
2. Prepare working dilution: Dilute the antibody in an appropriate diluent compatible with your assay (e.g., TBST + 5% non-fat milk for Western blot; PBS + 1% BSA for flow cytometry). Optimal dilution must be determined empirically by titration; start with a range such as 1:500–1:2000 for Western blot and 1:50–1:200 for immunofluorescence as a starting point, then optimise.
3. Blocking: Always include an appropriate blocking step to reduce non-specific background.
4. Incubation: Incubate with the primary antibody under conditions recommended in the datasheet (typically overnight at 4 °C for Western blot).
5. Secondary antibody: Use an anti-mouse IgG secondary antibody matched to your detection system, as p95 Mouse mAb is produced in mouse.
6. Controls: Include positive and negative controls in every experiment to validate results.

Note: All dilution ranges listed above are general starting points. Always validate working concentrations using your specific experimental system.

Troubleshooting Common Issues

If unexpected results are observed when using p95 Mouse mAb, consider the following troubleshooting steps:

Issue	Possible Cause	Suggested Action
No signal detected	Antibody concentration too low; antigen not expressed in sample	Increase antibody concentration; verify target expression in your cell/tissue model; check secondary antibody compatibility
High background	Insufficient blocking; antibody concentration too high	Extend blocking time; increase washing steps; reduce primary antibody concentration
Non-specific bands (Western blot)	Cross-reactivity; insufficient blocking	Optimise blocking buffer; reduce antibody concentration; confirm sample preparation conditions
Loss of activity after storage	Repeated freeze-thaw cycles; improper storage temperature	Aliquot upon receipt; store at −20 °C or −80 °C; avoid repeated freeze-thaw

Ordering and Technical Support

p95 Mouse mAb is available from Aladdin Scientific under catalog number Ab350605. To place an order or request additional technical information — including the full product datasheet, certificate of analysis, or application-specific guidance — please contact Aladdin Scientific customer service or visit the product page on the Aladdin website.

For bulk orders, custom conjugation inquiries, or specific lot reservations, reach out to the Aladdin sales team. Technical support scientists are available to assist with protocol optimisation and troubleshooting specific to your experimental system.

Frequently asked questions

What is the formulation of p95 Mouse mAb (Ab350605)?

p95 Mouse mAb (Ab350605, absea #KC-1341) is formulated in PBS (phosphate-buffered saline) only, without added carrier proteins such as BSA or glycerol. This PBS-only format provides flexibility for downstream conjugation and diverse assay formats.

How should p95 Mouse mAb be stored to preserve its activity?

For short-term use (up to one month), store at 4 °C. For long-term storage, aliquot the antibody into single-use volumes and store at −20 °C or −80 °C. Avoid repeated freeze-thaw cycles and prolonged exposure to light, as these can degrade antibody performance.

Which secondary antibody should be used with p95 Mouse mAb?

Because p95 Mouse mAb is produced in mouse, an anti-mouse IgG secondary antibody is required. Select a secondary antibody matched to your specific detection system (e.g., HRP-conjugated for Western blot, fluorophore-conjugated for immunofluorescence or flow cytometry).

What dilution should I use for p95 Mouse mAb in Western blot?

Optimal dilution must be determined empirically for each experimental system. As a general starting point for Western blot, a dilution range of 1:500 to 1:2000 is commonly used for mouse monoclonal antibodies. Always titrate to find the concentration that gives the best signal-to-noise ratio. Refer to the product datasheet for manufacturer-validated dilution recommendations.

Can p95 Mouse mAb be used for applications other than Western blot?

The specific validated applications for Ab350605 are detailed in the manufacturer's product datasheet, which should be consulted for confirmed application compatibility. General applications for monoclonal antibodies include Western blot, immunofluorescence, immunohistochemistry, flow cytometry, and ELISA, but suitability must be confirmed for each application individually.

References

1. Aladdin Scientific product page: p95 Mouse mAb, Cat. No. Ab350605
2. absea Biotechnology product reference: KC-1341, p95 Mouse Monoclonal Antibody

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.