S100A16 Mouse mAb: FAQs, Applications & Protocols

S100A16 Mouse mAb is a monoclonal antibody raised in mouse targeting the S100A16 calcium-binding protein, designed to support research into S100 family protein expression, localization, and function across multiple experimental applications.

Product SKU

Ab350701

Antibody Type

Mouse Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline)

Target Protein

S100A16 (S100 calcium-binding protein A16)

Reference Code

absea#KC-43929

Host Species

Mouse

What Is S100A16 and Why Is It Relevant in Research?

S100A16 is a member of the S100 family of calcium-binding proteins, a group characterized by two EF-hand calcium-binding motifs. S100 proteins are involved in the regulation of a variety of cellular processes including cell cycle progression, differentiation, apoptosis, and calcium homeostasis. S100A16 has attracted particular research interest due to its reported expression in multiple tissues and its potential roles in adipogenesis and cancer biology. Researchers investigating calcium signaling pathways, metabolic disorders, or tumor microenvironments may find S100A16 a relevant target. Targeting S100A16 with a specific monoclonal antibody such as Ab350701 enables reliable detection and characterization of this protein in experimental systems.

Product Specifications and Formulation Details

The S100A16 Mouse mAb (SKU: Ab350701) is a monoclonal antibody of mouse origin formulated in PBS (phosphate-buffered saline). The PBS-only formulation means the antibody solution does not contain stabilizers such as BSA or glycerol unless otherwise specified in the accompanying datasheet. Researchers should consult the product datasheet for confirmed details on concentration, clone identity, isotype, and validated applications. The reference code absea#KC-43929 may be used when cross-referencing this product with supplier documentation. As with all monoclonal antibodies in PBS-only format, proper handling and storage are critical to maintaining product integrity.

Recommended Storage and Handling

Because S100A16 Mouse mAb (Ab350701) is formulated in PBS without additional protein stabilizers, careful storage is essential to prevent degradation or loss of activity. General best practices for PBS-only monoclonal antibodies include: aliquoting the stock solution into single-use volumes upon receipt to avoid repeated freeze-thaw cycles; storing aliquots at -20°C or -80°C for long-term preservation; and keeping a working aliquot at 4°C for short-term use (typically up to 2-4 weeks). Avoid exposure to repeated temperature fluctuations and direct light. Always consult the product datasheet for manufacturer-specific storage recommendations, as these take precedence over general guidelines.

Typical Applications and Protocol Guidance

Monoclonal antibodies targeting S100 family proteins are commonly employed in a variety of immunological and biochemical assay formats. Potential applications for S100A16 Mouse mAb may include Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), and Enzyme-Linked Immunosorbent Assay (ELISA), subject to validation as confirmed on the product datasheet. For Western Blot, standard SDS-PAGE under reducing conditions is typically used, followed by membrane transfer and incubation with the primary antibody at the manufacturer-recommended dilution. For IHC, appropriate antigen retrieval methods should be optimized for the tissue type. Researchers are strongly advised to refer to the official product datasheet for validated applications, recommended dilutions, and positive control information before commencing experiments.

Troubleshooting Common Issues

Researchers using S100A16 Mouse mAb may occasionally encounter issues such as high background, weak signal, or non-specific bands. Below are general troubleshooting recommendations applicable to monoclonal antibody experiments:

• High background: Increase blocking time or concentration; optimize antibody dilution; ensure thorough washing steps.
• Weak or absent signal: Verify target expression in the chosen cell or tissue model; check antibody storage history for freeze-thaw damage; optimize antigen retrieval (for IHC/IF).
• Non-specific bands (WB): Titrate antibody concentration downward; include appropriate negative controls; confirm species cross-reactivity on the datasheet.
• Inconsistent results: Standardize sample preparation and lysis buffer conditions; ensure consistent incubation times and temperatures.

For persistent issues, contact Aladdin Scientific technical support with your experimental details and lot number for expert guidance.

Frequently asked questions

What is the formulation of S100A16 Mouse mAb (Ab350701)?

S100A16 Mouse mAb (Ab350701) is formulated in PBS (phosphate-buffered saline) only, without additional stabilizers such as BSA or glycerol. Researchers should aliquot upon receipt and store appropriately to maintain antibody activity. Please refer to the product datasheet for the most current formulation details.

Which applications has S100A16 Mouse mAb been validated for?

Validated applications for S100A16 Mouse mAb (Ab350701) are specified on the official product datasheet. Common applications for S100 family monoclonal antibodies include Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA, but confirmed application suitability must be verified on the datasheet before experimental use. Contact Aladdin Scientific technical support for application-specific guidance.

How should S100A16 Mouse mAb be stored to ensure optimal performance?

For long-term storage, aliquot S100A16 Mouse mAb upon receipt and store at -20°C or -80°C to avoid repeated freeze-thaw cycles. A working aliquot may be kept at 4°C for short-term use. As the formulation is PBS only, extra caution should be taken to prevent degradation. Always follow the storage instructions on the product datasheet.

What is the target protein of S100A16 Mouse mAb and what is its biological significance?

The target protein is S100A16, a member of the S100 family of calcium-binding proteins characterized by EF-hand motifs. S100A16 has been implicated in cellular processes such as calcium homeostasis, adipogenesis, and potentially in cancer biology. Its broad tissue expression makes it a relevant research target for scientists studying metabolism, cancer, and cell signaling.

Can S100A16 Mouse mAb be used with non-human samples, and what species reactivity should I expect?

Species reactivity for Ab350701 should be confirmed on the official product datasheet prior to use with non-human samples. While some S100 family antibodies demonstrate cross-reactivity with rat or other mammalian species due to protein sequence conservation, this cannot be assumed without datasheet validation. Contact Aladdin Scientific technical support for species cross-reactivity enquiries.

References

1. Aladdin Scientific Product Datasheet: S100A16 Mouse mAb, SKU Ab350701 (absea#KC-43929).
2. Donato R, et al. Functions of S100 proteins. Current Molecular Medicine. General reference for S100 protein family biology.
3. Bresnick AR, Weber DJ, Bhatt DL. S100 proteins in cancer. Nature Reviews Cancer. General reference.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.