Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Triphenylmethane dye-type indicators are a class of organic color-change reagents based on triarylmethane or related lactone/quinoid structures. They are widely used in acid-base titration, non-aqueous titration, complexometric titration, protein assays, histological staining, microbiological ...
In many laboratory workflows, reagents whose names include “blue” are used for tracking, staining, or colorimetric readouts. Despite similar naming, their molecular targets, signal-generation mechanisms, and susceptibility to interference differ substantially. Background elevation, unstable ...
Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?
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