Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
D-Luciferin is a small-molecule substrate commonly used in bioluminescent reactions—most famously those observed in fireflies. When oxidized in the presence of the enzyme luciferase, along with ATP and oxygen, D-Luciferin emits visible light, typically in the yellow-green range (~560 nm).
Updated Aug 2025·42 min read
Related topics
Techniques often explored alongside immunological experiments.
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