Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Secondary antibodies bind primary antibodies to allow detection, sorting and purification of target antigens. Since they are specific for the primary antibody species and isoform, they can detect the protein of interest. The need for a secondary antibody depends on your antibody detection method.
Updated Jun 2025·33 min read
Related topics
Techniques often explored alongside immunological experiments.
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