Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Chlorophyll is a class of green pigments broadly present in higher plants and other photosynthetic organisms, playing a central role in light harvesting and energy transfer during photosynthesis.
Glutathione (GSH) is one of the most important low-molecular-weight thiols in cells. It participates in reactive oxygen species (ROS) detoxification, maintains protein thiols in a reduced state, drives the glutathione peroxidase/reductase cycle, and—together with oxidized glutathione ...
IUPAC defines fluorescence as: a process in which an excited molecule spontaneously emits radiation while preserving the spin multiplicity (simplified: absorb light → emit light shortly after).
Quantum dots are tiny nanocrystals that glow when stimulated by an external light source, such as ultraviolet light. How many atoms are contained in the dots determines their size, and the size of the dots determines the color of the light emitted.
The fluorescence of a compound can be quenched, meaning it is reduced or completely switched off by processes that do not destroy the fluorophore permanently. Fluorescence quenching reduces the intensity of fluorescence, but the original intensity reappears as soon as the quencher is removed or ...
Besides the optical phenomena of refraction, scattering, interference, and diffraction in partially or totally transparent objects, which can result in colour impressions such as rainbows, sky blue, sparkling soap bubbles, gorgeous peacock feathers, or thin oil films and layers, the colours of ...
Techniques often explored alongside immunological experiments.
