DTNB

Get answers to common questions about Aladdin's Protein Cysteine Assay Kit (DTNB, Micro Method). Learn about the protocol, sensitivity, and sample prep.

Step-by-step protocol for using DTNB Solution (pH 8.0, 10 mM) to quantify free thiol groups via Ellman's reagent assay at 412 nm.

Cholinesterase detection systems are mainly used to measure the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and total cholinesterase (ChE). The differences among detection methods are mainly reflected in substrate type, signal generation mode, readout format, ability ...

Thiol and disulfide status are important chemical indicators for evaluating redox balance, protein structural stability, and the degree of stress-induced damage in biological samples. Measuring thiol content alone usually reflects only the level of reactive reduced species under a specific ...

Glutathione (GSH) is one of the most important low-molecular-weight thiols in cells. It participates in reactive oxygen species (ROS) detoxification, maintains protein thiols in a reduced state, drives the glutathione peroxidase/reductase cycle, and—together with oxidized glutathione ...

Ellman’s reagent, 5,5′-dithio-bis-(2-nitrobenzoic acid), also known as DTNB, is a water-soluble compound reacts with free sulfhydryl groups in solutions at slightly alkaline condition to yield a mixed disulfide and highly chromogenic 5-nitro-2- thiobenzoic acid (TNB) with molar extinction ...