Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Formaldehyde can undergo condensation, oxidation, or cyclization reactions with hydrazine derivatives, nitrogen-containing heterocyclic compounds, and aromatic chromogenic reagents to form colored products with characteristic visible-light absorption. Different colorimetric methods vary in ...
Fixation is a critical pretreatment step in histology, cytology, immunostaining, and ultrastructural observation. Its core purpose is to preserve the in situ morphology, molecular localization, and structural stability of samples as much as possible. Glutaraldehyde, paraformaldehyde, and ...
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