LC-MS/MS

Step-by-step protocol for handling, dissolving, and using IPPD-quinone-d5 as a deuterated internal standard in LC-MS/MS quantitation workflows.

Step-by-step protocol for preparing and using Ractopamine D6 (deuterium-labeled internal standard) in LC-MS/MS residue analysis workflows.

Step-by-step protocol for using Fleroxacin-d3 as a stable-isotope internal standard in LC-MS/MS quantification of fleroxacin in biological matrices.

Learn how Sulfadimethoxine-d4 is used as a deuterated internal standard for LC-MS/MS quantification of sulfonamide residues in food and biological matrices.

Perfluoro-n-octanoic acid (PFOA)-13C8 is a mass-labeled internal standard for accurate PFAS quantification by LC-MS/MS and GC-MS methods.

Guidelines for using Perfluoro-n-octanoic acid (PFOA)-13C8 as an isotope-labeled internal standard in PFAS quantification by LC-MS/MS and GC-MS methods.

Step-by-step protocol for using PFOS-13C8 (13C8-labeled PFOS sodium) as an isotope internal standard in LC-MS/MS quantification of perfluorooctanesulfonate.

Step-by-step protocol for preparing and using PFOS-13C8 (13C8-labeled sodium perfluorooctanesulfonate) as an internal standard in LC-MS/MS PFAS analysis.

Choline metabolism connects cell membrane phospholipid synthesis, methyl donor cycling, cholinergic neurotransmitter generation, hepatic lipid transport, and gut microbiota-related metabolism. Choline metabolism detection should not be limited to free choline alone. Depending on the research ...

The core of carbon-nitrogen metabolic crosstalk lies in the dynamic conversion between carbon skeletons and nitrogen flow. Glutamate and α-ketoglutarate form key hubs connecting the TCA cycle, transamination reactions, glutamine metabolism, and amino acid biosynthesis. Their changes reflect not ...

β-Hydroxybutyrate is one of the predominant ketone body forms in blood and tissues. It is commonly used to evaluate enhanced fatty acid oxidation, hepatic ketogenesis, starvation or fasting status, diabetic ketosis, exercise metabolism, and mitochondrial energy conversion. Compared with ...

TCA cycle metabolite detection is used to evaluate mitochondrial oxidative metabolism, substrate entry, anaplerotic reactions, redox status, and metabolic reprogramming. Common targets include citrate, α-ketoglutarate, succinate, fumarate, malate, and oxaloacetate. Method selection should be ...

Creatinine content detection is commonly used for renal function assessment, urinary indicator normalization, and drug nephrotoxicity studies in serum, plasma, urine, and animal experimental samples. The core differences among detection methods lie in reaction principle, signal generation mode, ...

Hydroxyproline determination is a classical quantitative technique in collagen-related research and is widely used in fibrosis assessment, tissue-remodeling studies, collagen-material analysis, and pharmacodynamic evaluation.