Stable isotope-labeled compounds use ²H, ¹³C, ¹⁵N, or ¹⁸O to label metabolic substrates, allowing their transformation and fate in vivo to be identified by mass spectrometry or nuclear magnetic resonance. This technology can be used to determine substrate sources, metabolic pathways, ...
Topic
metabolic flux
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
4 resources · protocols, FAQs & articles
TCA cycle metabolite detection is used to evaluate mitochondrial oxidative metabolism, substrate entry, anaplerotic reactions, redox status, and metabolic reprogramming. Common targets include citrate, α-ketoglutarate, succinate, fumarate, malate, and oxaloacetate. Method selection should be ...
Amino acid metabolism follows a core logic of “amino-group transfer—deamination release/assimilation—carbon-skeleton recovery—resynthesis and allocation”, spanning protein turnover, energy metabolism, and nitrogen homeostasis.
Vitamins, coenzymes, and enzymes together constitute the foundational system for cellular metabolic regulation and energy conversion.
Related topics
Techniques often explored alongside immunological experiments.