Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Loading control antibodies are used to detect stable reference proteins and provide internal references for target protein quantification, sample-to-sample comparison, evaluation of subcellular fractions, and multiplex detection. Their applicability depends on reference protein stability, sample ...
Since the first publication describing Western blotting, this immunodetection technique has been widely used to identify specific proteins in complex mixtures extracted from cells or tissues.
Updated Jun 2025·33 min read
Related topics
Techniques often explored alongside immunological experiments.
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