Does WB Primary Antibody Diluent contain sodium azide?
Does WB Primary Antibody Diluent contain sodium azide?
Topic
Primary antibody
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
7 resources · protocols, FAQs & articles
Immunohistochemical Antibody Selection Points
Choosing monoclonal or polyclonal antibodies? A single specific antibody produced by one type of B cell is called a monoclonal antibody. Monoclonal antibodies bind to a single specific antigenic determinant in a targeted manner, like a missile hitting a target with precision.
What is the concentration of the primary antibody?
What is the concentration of the primary antibody?
Protocol and tips for whole mount immunohistochemical staining
Whole mount staining is the staining of small pieces of tissue-usually embryos-without sectioning.
Antibody Isotype Controls
An isotype control antibody appears like a typical antibody but it does not attach itself to a specific target protein like the primary antibody used in the experiment.
Loading Controls for Western Blotting
Since the first publication describing Western blotting, this immunodetection technique has been widely used to identify specific proteins in complex mixtures extracted from cells or tissues.
IgG
IgG, the key factor of Humoral immunity response, is the main immunoglobulin in blood, lymph, cerebrospinal fluid and peritoneal fluid. IgG accounts for approximately 75% of total serum immunoglobulins and is the only immunoglobulin that can penetrate the human placenta. It is primarily ...
Related topics
Techniques often explored alongside immunological experiments.