Rt-qPCR

Nucleases are enzymes that catalyze the hydrolysis of phosphodiester bonds in DNA or RNA. They play fundamental roles in nucleic acid extraction, sample purification, contamination control, and molecular experiment quality control.

In real-time quantitative PCR (qPCR) and reverse-transcription real-time quantitative PCR (RT-qPCR), probe chemistries convert target amplification into quantifiable signals through “sequence-specific hybridization–fluorescence switching.” Compared with intercalating dye methods, ...

Rt-qPCR (real-time fluorescence quantitative PCR) is a basic experiment in molecular biology, which mainly includes three steps: RNA extraction, reverse transcription to cDNA, and real-time fluorescence quantitative PCR.