1,700+ technical articles by Aladdin Scientific's scientists on reagent selection, reaction mechanisms, assay design and formulation. New every week.
mTOR is a serine/threonine kinase and the catalytic subunit of the mTORC1 and mTORC2 complexes. mTORC1 is involved in energy homeostasis signaling. Akt phosphorylation regulates TSC2, inhibiting the activity of the TSC1/TSC2 heterodimer, relieving its suppression of mTORC1 and sustaining activation.
The Ras-Raf-MEK-MAPK-ERK signaling pathway plays a crucial role in cell differentiation, proliferation, and survival. This pathway transmits extracellular signals through ligands of receptor tyrosine kinases on the membrane, activating nuclear transcription factors that regulate gene expression ...
Janus kinases (JAKs) and signal transducers and activators of transcription (STATs) are two major components in the signaling cascade that transmit extracellular signals across the cell membrane and into the nucleus, thereby altering gene transcription.
The Hedgehog (Hh) signaling pathway is one of the core pathways regulating embryonic development in animals. Initially discovered in Drosophila, it plays a decisive role in embryogenesis and metamorphosis. In mammals, this pathway involves three homologous ligands: Sonic Hedgehog (SHH), Desert ...
Tyrosine kinases, such as anaplastic lymphoma kinase (ALK), have become critical targets in the development of novel chemotherapy and anti-inflammatory drugs. ALK plays a crucial role in brain development and also accelerates the onset and progression of various cancers, including anaplastic ...
D-Luciferin is a small-molecule substrate commonly used in bioluminescent reactions—most famously those observed in fireflies. When oxidized in the presence of the enzyme luciferase, along with ATP and oxygen, D-Luciferin emits visible light, typically in the yellow-green range (~560 nm).
Luciferase Enzyme Fact Sheet
Scientists may want to know the species from which our Luciferin is derived from. In general there are two kinds of Luciferin that are sold and they are referred to as Synthetic Firefly Luciferin or Synthetic Beetle Luciferin.
The three primary analogues of coelenterazine—Coelenterazine, Coelenterazine h, and Coelenterazine 400a—can all be employed in similar experimental settings. However, depending on the application, some analogues may yield more optimal results than others.