1,700+ technical articles by Aladdin Scientific's scientists on reagent selection, reaction mechanisms, assay design and formulation. New every week.
Bioorthogonal chemistry refers to a series of reactions that occur in a biological environment without affecting biomolecules or interfering with biochemical processes. For this purpose, the reaction must meet the following requirements: fast, efficient, and specific. • pH: The reaction must ...
In neoplastic cells, the de novo synthesis of nucleotides is responsible for maintaining sufficient nucleotide libraries to support DNA replication and RNA production. The metabolic pathways that support nucleotide production are dependent on metabolic intermediates provided by glycolysis and ...
Dialysis is the process of selective diffusion of substances through membranes. It can be used to separate solutes with different molecular weights. Substances with molecular weights lower than the membrane interception threshold can diffuse through the membrane, while substances with molecular ...
The ready to use dialysis bag is usually a bag shaped container made of a semi permeable membrane. During the preparation of biological macromolecules, dialysis technology is used to remove salt, a small amount of organic solvent, biological small molecule impurities, and concentrate samples. It ...
Chromatographic column is a widely used method in chromatography, and it is also one of the commonly used separation methods in organic experiments. This column is the main part of gel chromatography technology, and glass tube or plexiglass tube is generally used. A chromatographic method for ...
User Manual of Ready to use RC Membrane Dialysis Bag
Because the dialysis membrane is composed of spongy cross-linked polymers, the molecular weight cutoff (MWCO) representing the pore size of the membrane is an indirect parameter to measure the membrane separation performance. More precisely, the molecular weight of the membrane can be defined as ...
Generally, the semi permeable membrane is made into a bag, the sample solution of biomacromolecule is put into the bag, and the dialysis bag is immersed in water or buffer solution. The biomacromolecule with large molecular weight in the sample solution is retained in the bag, while the salt and ...
According to the different binding groups, Ni2+affinity chromatography columns can be divided into two types: Ni IDA agarose gel and Ni NTA agarose IDA and NTA.png gel.