When extracting proteins from suspension cells, can I add the product directly to boil and denature them?
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When extracting proteins from suspension cells, can I add the product directly to boil and denature them?
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When extracting proteins from suspension cells, can I add the product directly to boil and denature them?
Yes, it is possible to boil directly with 1× buffer, but it should be noted that the protein sample buffer obtained after this operation cannot be used for BCA concentration measurement.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "When extracting proteins from suspension cells, can I add the product directly to boil and denature them?" Aladdin Knowledge Base, updated 09.06.2025. https://www.aladdinsci.com/eu_de/faqs/when-extracting-proteins-from-suspension-cells-en.html
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