β-Glucosidase, CAS No.9001-22-3

CAS: 9001-22-3 Cat. No.: G755171 EC Number: 232-589-7

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥10U/mg powder; 10-60 U/mg protein

Bioactivity

≥10U/mg powder; 10-60 U/mg protein

Overview

Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking twoglucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is oneof the cellulases, enzymes involved in the decomposition of cellulose and relatedpolysaccharides; more specifically, an exocellulase with specificity for a variety ofbeta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducingresidues in beta-D-glucosides with release of glucose.

Applications

This enzyme is useful for structural investigations of carbohydrates and for theenzymatic determination of α-amylase when coupled with α-glucosidase in clinicalanalysis.

Product Information

Source: Sweet almond

Appearance: Light yellow amorphous powder, lyophilized

Form: Freeze dried powder

Molecular Weight:approx. 110 kDa

Activity: 10U/mg-solid or more (containing approx. 50% of BSA)

Contaminants: α-Amylase < 5.0×10⁻⁴%

Isoelectric point: 7.3

pH Stability: pH 6.0-9.0 (25°C, 64hr)

Optimum pH: 5.5

Thermal stability: below 50°C (pH 7.3, 1hr)

Optimum temperature: 50-55°C

Michaelis Constant: 2.8×10⁻³M (p-Nitrophenyl-ß-D-glucopyranoside), 3.3×10⁻³M (2,4-Dichlorophenyl-ß-D-glucopyranoside)

Structure: 2 subunits per mol of enzyme

Stabilizers: Bovine serum albumin (BSA), glutathione (reduced)
Stability: Stable at-20°C for at least 6 months (A decrease in activity of ca. 10% may occur at5°C within 6 months)

Specifications

Product Name

β-Glucosidase, CAS No.9001-22-3

Synonyms

beta-glucosidase | amygdalase | beta-D-glucoside glucohydrolase | cellobiase | gentobiase | β-D-Glucoside glucohydrolase | b-D-Glucosidase | b-D-Glucoside glycohydrolase | EC 3.2.1.21 | gentiobiase | emulsin | elaterase | aryl-beta-glucosidase | beta-D-gl

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥10U/mg powder; 10-60 U/mg protein

Bioactivity

≥10U/mg powder; 10-60 U/mg protein

Predicted molecular weight

~110 kDa

CAS

9001-22-3

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥10U/mg powder; 10-60 U/mg protein

Reconstitution

Reconstitute in ddH2O.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (2 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

The amount of enzyme which catalyzes the formation of one micromole of p-Nitrophenol per minute at 37°C.

References

1. Shi Cong-Rong, Zhang Xiao-Juan, Xie Han, Zhang Wei, Tang Rui-Xin, Lu Zhen-Ming, Geng Yan, Lu Ran, Wang Fang-Zhou, Chai Li-Juan, Zhang Hui-Ling, Xu Hong-Yu, Shi Jin-Song, Xu Zheng-Hong.  (2026)  Metabolomic profiling of goji fermentation: enhancing functional metabolites with high-glucose-adaptive LAB.  npj Science of Food,      [PMID:] [10.1038/s41538-026-00791-x]