High ROX Reference Dye (50×) - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,50×

Cat. No.: FP1509095

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 50×

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR, qPCR

Übersicht

High ROX Reference Dye (50×) is an inert fluorescent dye used in real-time quantitative PCR (qPCR) or RT-PCR to correct for well-to-well variations in fluorescence signals. It does not participate in the PCR reaction, and its fluorescence signal remains stable throughout the process. ROX dye can correct for fluorescence signal variations caused by factors such as uneven illumination, optical differences between wells (e.g., droplets, bubbles), and variations in reaction system composition. 

Instruments requiring High ROX Reference Dye (50×):

ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus.

Instruments requiring Low ROX Reference Dye (50×):

ABI 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6, 7, 12k Flex.

Stratagene MX3000P, MX3005P, MX4000P.

Instruments not requiring ROX correction:

Bio-Rad CFX96, CFX384, iCycler iQ, iQ 5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4.

Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96.

Eppendorf Mastercycler ep realplex, realplex 2 s.

Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000.

Thermo Scientific PikoReal Cycler.

Cepheid SmartCycler.

Illumina Eco qPCR.

Instructions for Use

Preparation of qPCR system using High ROX Reference Dye (50×):

For a 20 µL qPCR reaction system, add 0.4 µL of High ROX Reference Dye (50×).

Note:

• The dye can be added proportionally according to the total reaction volume.

• When preparing a multi-well system, it is recommended to prepare a master mix, ensuring thorough mixing before use.

Precautions

1. After receiving the reagent, it can be aliquoted for use to avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Spezifikationen & Reinheit

BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,50×

Stabilität und Lagerung

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot. Store in the dark.

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology

Chemische und physikalische Eigenschaften

• Empfindlichkeit: Light-sensitive

Häufig gestellte Fragen

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does DNase, RNase free mean?

DNase, RNase free indicates that the product has been processed and tested for sensitive nucleic acid and protein workflows. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

How should this product be stored?

Store at ?20 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.