BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
λ Bacteriophage is one of the earliest used cloning vectors, with a genome consisting of a double-stranded DNA molecule of approximately 50 kb. λ Phage exhibits two life cycles within the host cell: Lytic growth: The circular DNA replicates multiple times within the cell, synthesizing a large number of phage gene products, which assemble into phage particles. The host cell is then lysed to release progeny phages for the next round of infection. Lysogenic growth: The λ phage DNA integrates into the host chromosomal DNA, replicating along with the host genome and being passed on to daughter cells without causing host cell lysis. Researchers often exploit the lytic growth characteristics of λ phage to culture large quantities of phage particles and extract their DNA. Phage vectors are widely used in library screening. Following the acquisition of phage particles, λ DNA is extracted for downstream applications such as sequencing. A typical extraction procedure involves: centrifuging the λ phage lysate culture and collecting the supernatant; first, digesting residual host DNA/RNA with a mixture of RNase A and DNase I; then precipitating and collecting phage particles; extracting DNA via phenol-isoamyl alcohol treatment; centrifuging to remove residual debris; and finally performing rapid washing and centrifugation steps to eliminate salts, cellular metabolites, proteins, and other impurities. The resulting DNA yield is relatively high, though the purity is moderate, which is generally sufficient for most molecular biology experiments. This product is a convenient kit for λ phage genomic DNA extraction. The principle is based on PEG precipitation and phenol-isoamyl alcohol extraction. After centrifugation to remove residual debris, followed by rapid washing and centrifugation steps to remove salts, metabolites, and proteins, the λ phage genomic DNA is obtained. The product can be used in downstream applications such as restriction enzyme digestion and PCR. This kit is intended for research use only and is not suitable for clinical diagnosis or other applications.
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