GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Glutamate dehydrogenase (GDH) is widely distributed in organisms and plays an important role in ammonia assimilation and the metabolism of organic nitrogen compounds. This enzyme utilizes NADPH or NADH as coenzymes; both coenzyme forms exist in animals and plants, but in yeast, the NADPH-dependent glutamate dehydrogenase (NADPH-GDH, EC 1.4.1.4) is predominant. This kit provides a rapid and sensitive assay: NADPH-GDH specifically catalyzes the oxidative deamination of glutamate to α-ketoglutarate and NADPH (reverse reaction). The generated NADPH reacts with a specific chromogenic reagent to produce a yellow product with a maximum absorbance peak at 450 nm. By continuously monitoring the initial linear increase in OD₄₅₀ per unit time (ΔOD₄₅₀/min) in kinetic mode, the NADPH-GDH enzyme activity in the sample can be precisely calculated.
Reaction equation:
L-Glutamate + NADP⁺ + H₂O ⟶ α-Ketoglutarate + NH₄⁺ + NADPH + H⁺
Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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