PCNA Mouse mAb (AF594) - Primärer Antikörper, specific to PCNA

Cat. No.: Ab219144

GRADE & PURITY ExactAb™, Validated, 0.5 mg/mL

Application

IF/ICC

Host species

Maus

Reactive species

Maus, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

C04/3D7

Conjugation

AF594

Übersicht

Mouse anti Human PCNA Antibody, Monoclonal (C04/3D7), could be used for IF/ICC.

Specifications

Product Name

PCNA Mouse mAb (AF594) - Primärer Antikörper, specific to PCNA

Synonyme

ATLD2 antibody | cb16 antibody | Cyclin antibody | DNA polymerase delta auxiliary protein antibody | etID36690.10 antibody | fa28e03 antibody | fb36g03 antibody | HGCN8729 antibody | MGC8367 antibody | Mutagen-sensitive 209 protein antibody | OTTHUMP00000

Spezifikationen & Reinheit

ExactAb™,Validated,0.5 mg/mL

Biochemische und physiologische Mechanismen

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote

Wirtsspezies

Maus

Spezifität

PCNA

Immunogen

Full length recombinant human PCNA

Positive Kontrolle

IF/ICC: Hela cells

Konjugation

AF594

Anregung (nm)

Yellow-Green Laser(594nm)

Emission (nm)

618nm

Ex/Em(nm)

Ex:590nm;Em:618nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein A purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Bilder

IF/ICC: PCNA Mouse mAb (AF594) (Ab219144)

PCNA Mouse mAb (AF594) (Ab219144) - IF/ICC 

IF analysis of PCNA (Red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (AF594) (Ab219144) at a dilution of 1/500 in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Zugehörige Ziele (menschlich)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)

Discover Target: PCNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PCNA Mouse mAb (AF594)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.