GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥30 U/mg enzyme powder
EnzymoPure™, Recombinant, ≥30 U/mg enzyme powder Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Phospholipase C (PLC) from Bacillus cereus is useful for enzymatic determination of lecithin when coupled with alkaline phosphatase and choline oxidase.
Preparation and Specification
Appearance: White to brownish amorphous powder, lyophilized
Specific activity: ≥30 U/mg enzyme powder
Properties
Molecular weight: 20 kDa (Sephadex G–100)
Isoelectric point: pH 7.0
Michaelis constants: Phosphatidylcholine 2.0 × 10⁻² M
Optimum pH: 7.0–9.0
pH stability: 5.0–10.0 (in 0.1% BSA)
Thermal stability: Stable at 55°C and below (pH 7.5, 10 min)
Storage stability: At least one year at -20°C
Activator: Ca²⁺, Sodium deoxycholate
Inhibitor: Tris–HCl buffer
Specifications
Product Name
Phospholipase C (PLC) from Bacillus cereus, CAS No.9001-86-9
Grade
Recombinant, EnzymoPure™
Specifications & Purity
EnzymoPure™, Recombinant, ≥30 U/mg enzyme powder
CAS
9001-86-9
Enzyme Commission Number
EC 3.1.4.3
Molecule Type
Enzyme
Storage and Shipping
Concentration
≥30 U/mg enzyme powder
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Unit definition
One unit is defined as the amount of enzyme which liberates 1 μmole of phosphoryl choline per minute at 37℃ under the conditions specified in the assay procedure.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
1.Xuefeng Li, Wenlong Yuan, Lin Mei, Yufei Zhang, Lei Ping, Mingming Zheng, Shengjiao Yu, Xi Du, Jun Wei. (2026) Enzymatic Degumming of Arachidonic Acid Oil Using Immobilized Phospholipase A1 on Hollow Double-Layer Mesoporous Silica Nanoparticles. MOLECULES, 31 (5):(816). [PMID:41828804][10.3390/molecules31050816]
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Reviews
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