Recombinant CREB Antibody - Primärer Antikörper, specific to CREB1, Rabbit IgG

Cat. No.: Ab097670

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.2 mg/mL

Application

IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human CREB Antibody, Recombinant, could be used for WB, IHC and so on.

Application:
WB: 1/500-1/1000
IHC: 1/50-1/100

Protein function
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

Specifications

Product Name

Recombinant CREB Antibody - Primärer Antikörper, specific to CREB1, Rabbit IgG

Synonyme

active transcription factor CREB | cAMP responsive element binding protein 1 | cAMP-response element-binding protein-1 | cAMP-responsive element-binding protein 1 | CREB | CREB1 | CREB-1 | cyclic AMP-responsive element-binding protein 1 | MGC9284 | transa

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Wirtsspezies

Kaninchen

Spezifität

CREB1

Immunogen

A synthetic peptide derived from human CREB (AA 260-278).

Positive Kontrolle

WB: HeLa, Jurkat, K562, Hep G2, A549, A431, HEK293, RAW 264.7 and C6 cell lysates. IHC: Human lung cancer tissue.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Immunogen affinity purified

Konzentration

0.2 mg/mL

Hinweis

This product is about to be sold out and discontinued. The replacement product is Ab222289

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant CREB Antibody (Ab097670)

Recombinant CREB Antibody (Ab097670) - Western Blot
All lanes: Recombinant CREB Antibody (Ab097670) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 47 kDa
Exposure time: 29.1 s

IHC: Recombinant CREB Antibody (Ab097670)

Recombinant CREB Antibody (Ab097670) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant CREB Antibody (Ab097670). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• Flow Cytometry:

  5μl/test

  For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Zugehörige Ziele (menschlich)

CREB1 Tbio Cyclic AMP-responsive element-binding protein 1 (0 Activities)

Discover Target: CREB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CREB Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.