Recombinant GNB2 Antibody - Primärer Antikörper, specific to GNB2, Rabbit IgG

Cat. No.: Ab105941

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human GNB2 Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/500 - 1/1000

IHC: 1/50-1/100

IF/ICC: 1/200

Protein Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Specifications

Product Name

Recombinant GNB2 Antibody - Primärer Antikörper, specific to GNB2, Rabbit IgG

Synonyme

G protein beta 2 subunit antibody | G protein subunit beta 2 antibody | G protein subunit beta-2 antibody | GBB2_HUMAN antibody | Gnb2 antibody | Gnb2l1 antibody | Guanine nucleotide binding protein beta 2 subunit antibody | Guanine nucleotide binding pro

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Wirtsspezies

Kaninchen

Spezifität

GNB2

Immunogen

A synthetic peptide derived from human GNB2 (AA 42-55)

Positive Kontrolle

WB: Jurkat, MCF7, C6, NIH/3T3, HeLa and SK-N-SH cell lysates. IHC: Human cholangiocarcinoma tissue. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Antigen affinity purified

Konzentration

0.2 mg/mL

Product Line

biological_products

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant GNB2 Antibody (Ab105941)

Recombinant GNB2 Antibody (Ab105941) - Western Blot
All lanes: Recombinant GNB2 Antibody (Ab105941) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 37 kDa
Observed band size: 32 kDa
Exposure time: 50.7 s

IHC: Recombinant GNB2 Antibody (Ab105941)

Recombinant GNB2 Antibody (Ab105941) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant GNB2 Antibody (Ab105941). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant GNB2 Antibody (Ab105941)

Recombinant GNB2 Antibody (Ab105941) - IF/ICC

IF analysis of Recombinant GNB2 Antibody (Ab105941) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant GNB2 Antibody (Ab105941) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Anwendung

Anwendung

Dilution info

WB

1/500-1/1000

IHC

1/50 - 1/100

Zugehörige Ziele (menschlich)

GNB2 Tbio Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (0 Activities)

Discover Target: GNB2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant GNB2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.