Recombinant PP2A alpha/beta Antibody - Primärer Antikörper, specific to PPP2CA/PPP2CB, Rabbit IgG

Cat. No.: Ab122709

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Hamster, Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human PP2A alpha/beta Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Specifications

Product Name

Recombinant PP2A alpha/beta Antibody - Primärer Antikörper, specific to PPP2CA/PPP2CB, Rabbit IgG

Synonyme

PPP2CA | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | PP2A-alpha | Replication protein C | RP-C | PP2A A antibody | PP2A alpha antibody | PP2A B antibody | PP2A beta antibody | PP2A-alpha antibody | PP2A-beta antibody | PP2AA_

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Wirtsspezies

Kaninchen

Spezifität

PPP2CA/PPP2CB

Immunogen

A synthetic peptide derived from human PP2A alpha/beta (AA 277-294).

Positive Kontrolle

WB: HeLa, C6, NIH/3T3, A431, Jurkat, HEK293, A549 and Raw264.7 cell lysates. IHC: Human lung cancer. ICC/IF: HeLa cells.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Immunogen affinity purified

Konzentration

0.2 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant PP2A alpha/beta Antibody (Ab122709)

Recombinant PP2A alpha/beta Antibody (Ab122709) - Western Blot
All lanes: Recombinant PP2A alpha/beta Antibody (Ab122709) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 34 kDa
Exposure time: 10.0 s

IF: Recombinant PP2A alpha/beta Antibody (Ab122709)

Recombinant PP2A alpha/beta Antibody (Ab122709) - IF
Immunocytochemistry analysis of PP2A alpha/beta (green) in HeLa cells using Recombinant PP2A alpha/beta Antibody (Ab122709) and cells were counterstained with DAPI (blue).

IHC: Recombinant PP2A alpha/beta Antibody (Ab122709)

Recombinant PP2A alpha/beta Antibody (Ab122709) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant PP2A alpha/beta Antibody (Ab122709). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

Zugehörige Ziele (menschlich)

PPP2CA Tchem Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (0 Activities)

Discover Target: PPP2CA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PPP2CB Tbio Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (0 Activities)

Discover Target: PPP2CB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PPP2CB Tbio Serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform (1 Activities)

Discover Target: PPP2CB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant PP2A alpha/beta Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.