ELISA

Step-by-step protocol for preparing and using Wash Buffer II in immunoassay and molecular biology workflows to minimize background and nonspecific binding.

Step-by-step milk protein blocking and detection protocol for Western blot and ELISA. Tips on preparation, concentration, and troubleshooting for reproducible results.

Step-by-step guideline for One-Component TMB Substrate Solution (Ultra-Sensitivity): protocol, optimization, storage, and troubleshooting for HRP-based ELISA assays.

Step-by-step ELISA protocol for TMB Supersensitive Liquid Substrate (T755518). Covers preparation, incubation, stop solution, and plate reading for sensitive HRP detection.

Step-by-step protocol and technical guide for High Sensitivity TMB Chromogen Solution for ELISA (H743375). Optimize sensitivity, storage, and troubleshooting.

Step-by-step guidelines for using Rat IgG2b Mouse mAb (HRP) Ab349244 in ELISA, WB, and IHC workflows for optimal detection results.

Step-by-step protocol for PON3 Mouse mAb (Ab350645): recommended dilutions, storage, and western blot/ELISA applications for PON3 research.

Step-by-step protocol for Rat IgG2c Mouse mAb (HRP) Ab350678: optimal dilutions, storage, and detection in ELISA and Western blot.

Learn how CDP-Star, a 1,2-dioxetane chemiluminescent substrate, enables ultrasensitive alkaline phosphatase detection in blotting and ELISA assays.

Blocking is a critical step for controlling nonspecific background in immunoassays such as Western Blot, ELISA, IHC, and IF, but the targets of blocking are not completely identical among different experiments. BSA, Casein, and protein-free blocking solutions are mainly used to limit nonspecific ...

Albumin is a frequently used protein material in biological samples, cell culture, immunoassays, and drug-binding studies. Its core value comes from its good water solubility, stability, ligand-binding capacity, and carrier function. In research applications, serum albumin, bovine serum albumin ...

The experimental value of lectin-based detection lies in converting glycan recognition into signals that can be localized, compared, and validated. Histochemistry, ELISA, and Western blot correspond to three different levels of information output: spatial distribution analysis, solid-phase ...

Lipoproteins are spherical particles composed of a hydrophobic lipid core and a hydrophilic surface shell. The core is enriched in hydrophobic lipids such as cholesteryl esters and triglycerides, whereas the surface is formed by phospholipids, free cholesterol, and apolipoproteins, enabling ...

Nipah virus (NiV) is an emerging zoonotic pathogen characterized by high pathogenicity and the capacity to cause sporadic cases and localized outbreaks in South and Southeast Asia.

Enzyme-linked immunosorbent assay (ELISA) is a highly sensitive quantitative detection method based on the specific reaction between antigen and antibody, and is widely used in immunology, molecular biology, drug development, and clinical diagnosis. Because the detection signal of this technique ...

Can ECL be used in ELISA?

The Interleukin (IL) family is a group of cytokines and development factors mainly derived from white blood cells, which play a key role in coordinating the immune response.

Antibodies are powerful research tools used in a variety of laboratory techniques. Here, we will provide a brief overview of the most popular laboratory techniques, focusing on how they use antibodies.