Recombinant RAB8A Antibody - Primärer Antikörper, specific to RAB8A, Rabbit IgG

Cat. No.: Ab124262

GRADE & PURITY Recombinant, Validated, ExactAb™, 1.5 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human RAB8A Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function:
May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.

Specifications

Product Name

Recombinant RAB8A Antibody - Primärer Antikörper, specific to RAB8A, Rabbit IgG

Synonyme

MEL antibody | Mel transforming oncogene (derived from cell line NK14)RAB8 homolog antibody | Mel transforming oncogene (derived from cell line NK14) antibody | Mel transforming oncogene (RAB8 homolog) antibody | Mel transforming oncogene antibody | MGC12

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 1.5 mg/mL

Wirtsspezies

Kaninchen

Spezifität

RAB8A

Immunogen

A synthetic peptide derived from human RAB8A (AA 171-184)

Positive Kontrolle

WB: K562, HeLa, Hep G2, MCF7, RAW 264.7 and C6 cell lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Antigen affinity purified

Konzentration

1.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant RAB8A Antibody (Ab124262)

Recombinant RAB8A Antibody (Ab124262) - Western Blot
All lanes: Recombinant RAB8A Antibody (Ab124262) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 26 kDa
Exposure time: 8.1 s

IF/ICC: Recombinant RAB8A Antibody (Ab124262)

Recombinant RAB8A Antibody (Ab124262) - IF/ICC
Immunocytochemistry analysis of Rab8A (green) in HeLa using Recombinant RAB8A Antibody (Ab124262) and DAPI (blue).

IHC: Recombinant RAB8A Antibody (Ab124262)

Recombinant RAB8A Antibody (Ab124262) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant RAB8A Antibody (Ab124262). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

Zugehörige Ziele (menschlich)

RAB8A Tbio Ras-related protein Rab-8A (0 Activities)

Discover Target: RAB8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant RAB8A Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.